| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We focused on Lefty, the protein identified by shotgun proteomics analysis. Lefty expression level was significantly higher in OCCCas compared with non-OCCCas. OCCCa cells stably overexpressing Lefty reduced cell proliferation, along with decreased pSmad2 expression and either activated p53/p21waf1 pathway or increased p27kip1 expression directly or indirectly. Moreover, treatment of the stable cells with cisplatin exhibited increased apoptotic cells, together with inhibition of pSmad2-mediated XIAP expression and decreased ratio of bcl2/bax. In clinical samples, a significantly higher number of apoptotic cells and lower Ki-67 LI were observed in OCCCa with high Lefty score relative to those with low score. These findings suggest that Lefty may be an excellent OCCCa-specific molecular marker, which has anti-tumor effects altering cell proliferation and susceptibility to apoptosis.
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