| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we examined invasion-associated microRNAs in the stroma of lung adenocarcinoma by microarray analysis of the microRNA after microdissection from invasive and non-invasive part of lung adenocarcinoma. To investigate the significance of each microRNA (mainly miR-21 and miR-199) in cancer associated fibroblasts (CAFs) in the lung adenocarcinoma, in situ hybridization targeting each microRNA was applied to invasive adenocarcinoma. MiR-21 was highly expressed in cancer cells and CAFs, respectively. Lung adenocarcinoma cases with miR-21 high CAFs showed lower survival than those with miR-21low CAFs. The cell line study suggested that miR-21 in CAFs was a driving force to promote progression of lung adenocarcinoma through the interaction with calumenin which was a novel colon CAF secreted protein.
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