Study of the abnormal expression and function of stromal microRNAs associated with progression of lung cancer
Project/Area Number |
26860248
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Human pathology
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Research Institution | The University of Tokyo (2015-2016) Teikyo University (2014) |
Principal Investigator |
Shigeki Morita 東京大学, 医学部附属病院, 助教 (30707021)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | microRNA / 肺癌 / 間質 / マイクロダイセクション / in situ hybridization / 癌関連線維芽細胞 / miR-21 / calumenin |
Outline of Final Research Achievements |
In this study, we examined invasion-associated microRNAs in the stroma of lung adenocarcinoma by microarray analysis of the microRNA after microdissection from invasive and non-invasive part of lung adenocarcinoma. To investigate the significance of each microRNA (mainly miR-21 and miR-199) in cancer associated fibroblasts (CAFs) in the lung adenocarcinoma, in situ hybridization targeting each microRNA was applied to invasive adenocarcinoma. MiR-21 was highly expressed in cancer cells and CAFs, respectively. Lung adenocarcinoma cases with miR-21 high CAFs showed lower survival than those with miR-21low CAFs. The cell line study suggested that miR-21 in CAFs was a driving force to promote progression of lung adenocarcinoma through the interaction with calumenin which was a novel colon CAF secreted protein.
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Report
(4 results)
Research Products
(9 results)
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[Presentation] A retrospective analysis of patients with small lung adenocarcinoma (<2cm) by new World Health Organization classification2016
Author(s)
Keita Nakao, Jun-ichi Nitadori, Shigeki Morita, Hideki Kuwano, Kazuhiro Nagayama, Masaki Anraku, Aya Shinozaki-Ushiku, Masaaki Sato, Masashi Fukayama, Jun Nakajima
Organizer
IASLC 17th World conference on lung cancer
Place of Presentation
Vienna, Austria
Year and Date
2016-12-04
Related Report
Int'l Joint Research
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