The mechanism of influenza virus internalization into host cells via calcium signaling-mediated endocytosis
Project/Area Number |
26860254
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Hokkaido University |
Principal Investigator |
Fujioka Yoichiro 北海道大学, 医学(系)研究科(研究院), 助教 (70597492)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | エンドサイトーシス / インフルエンザウイルス / イメージング / シグナル伝達 / カルシウム / ウイルス / FRET |
Outline of Final Research Achievements |
We have reported that Ras-PI3K signaling mediates endocytosis, and influenza viruses exploit this pathway to expedite their efficient incorporation into cells. Moreover, calcium signaling, triggered by the viruses, has been identified as an upstream regulator of Ras-PI3K signaling. In this study, we explored in detail the mechanism by which calcium signaling is activated upon the viral infection. First, we performed high-speed imaging experiments with fluorescently labeled virus particles to gain further insight into the spatiotemporal dynamics of Ca2+ responses upon virus entry. Immediately after infection, localized and modest Ca2+ elevations, prior to the aforementioned robust Ca2+ release from endoplasmic reticulum, were detected in the regions where the labeled particles were adsorbed. Moreover, we identified the membrane protein, which is involved in the Ca2+ elevation by the viruses.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers2016
Author(s)
T.Yamada, M. Tsuda, T. Wagatsuma, Y. Fujioka, M. Fujioka, A. O. Satoh, K. Horiuchi, S. Nishide, A. Nanbo, Y. Totsuka, H. Haga, S. Tanaka, M. Shindoh, and Y. Ohba
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 23545-23545
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A role of the sphingosine-1-phosphate (S1P)- S1P receptor 2 pathway in Epithelial Defense Against Cancer (EDAC)2015
Author(s)
Yamamoto, S., Yako, Y., Fujioka, Y., Kajita, M., Kameyama, T., Kon, S., Ishikawa, S., Ohba, Y., Ohno, Y., Kihara, A., and Fujita, Y.
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Journal Title
Molecular Biology of the Cell
Volume: 27(3)
Issue: 3
Pages: 491-499
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Agonist-promoted ubiquitination differentially regulates receptor trafficking of endothelin type A and type B receptors.2014
Author(s)
Terada K., Horinouchi T., Fujioka Y., Higashi T., Nepal P., Horiguchi M., Sarita K., Hatate C., Hoshi A., Harada T., Mai Y., Ohba Y. and Miwa S.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 51
Pages: 35283-35295
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] 生化学2015
Author(s)
藤岡容一朗、大場雄介
Total Pages
10
Publisher
日本生化学会
Related Report
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