Identification of cellular mRNAs targeted to mRNPs during infection
Project/Area Number |
26860256
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Gunma University |
Principal Investigator |
Seto Eri 群馬大学, 大学院医学系研究科, 助教 (40431382)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | P-body / mRNP / Trypanosoma cruzi / 感染応答 / P-body / Stress granule |
Outline of Final Research Achievements |
P-bodies are cytoplasmic mRNP granules involved in translation and degradation of mRNAs. In this study, we investigated the effect of Trypanosoma cruzi(T. cruzi)infection on P-body assembly in host cells, and found that the number of P-body foci dramatically increased within 24h post-infection. RNAi-mediated knockdown of P-bodies significantly enhanced the infectivity and growth of T. cruzi. It was therefore expected that accumulated P-bodies may positively regulate the expression of mRNAs required for anti-parasitic immune responses. To identify cellular mRNAs targeted to P-bodies during infection, we purified P-bodies using immunoprecipitation, and analyzed the coimmunoprecipitated mRNAs by deep sequencing.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] Inhibition of autolysosome formation in host autophagy by Trypanosma cruzi infection.2017
Author(s)
Onizuka, Y., Takahashi, C., Uematsu, A., Shinjo, S., Seto, E., and Nalajima-Shimada, J.
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Journal Title
Acta Tropica
Volume: 21
Pages: 57-62
DOI
Related Report
Peer Reviewed
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