| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
P-bodies are cytoplasmic mRNP granules involved in translation and degradation of mRNAs. In this study, we investigated the effect of Trypanosoma cruzi（T. cruzi）infection on P-body assembly in host cells, and found that the number of P-body foci dramatically increased within 24h post-infection. RNAi-mediated knockdown of P-bodies significantly enhanced the infectivity and growth of T. cruzi. It was therefore expected that accumulated P-bodies may positively regulate the expression of mRNAs required for anti-parasitic immune responses. To identify cellular mRNAs targeted to P-bodies during infection, we purified P-bodies using immunoprecipitation, and analyzed the coimmunoprecipitated mRNAs by deep sequencing.
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