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Clarification of mechanism in induction of DNA methylation using Epstein-Barr virus infection experiment

Research Project

Project/Area Number 26860257
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionChiba University

Principal Investigator

Matsusaka Keisuke  千葉大学, 医学(系)研究科(研究院), 助教 (40610150)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエピジェネティクス / 胃癌 / Epstein-Barr virus
Outline of Final Research Achievements

Genome-wide DNA methylation analysis in early phase of Epstein-Barr virus infection revealed how aberrant DNA methylation could be induced around transcription start sites (TSSs) or in genebody and intergenic regions. DNA methylation occurred from the CpG shore or shelves to CpG islands. In expression analysis by RNA-sequencing, dynamic expression alteration of both host cells and EBV could be observed. These findings should be significant clues to elucidate the mechanisms of induction of aberrant DNA methylation.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Genetic and epigenetic aberrations occurring in colorectal tumors associated with serrated pathway.2016

    • Author(s)
      Sakai E, Fukuyo M, Ohata K, Matsusaka K, Doi N, Mano Y, Takane K, Abe H, Yagi K, Matsuhashi N, Fukushima J, Fukayama M, Akagi K, Aburatani H, Nakajima A, Kaneda A.
    • Journal Title

      Int J Cancer

      Volume: 138 Issue: 7 Pages: 1634-44

    • DOI

      10.1002/ijc.29903

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of 18F-FDG uptake for detecting lymph node metastasis of gastric cancer: a prospective pilot study for one-to-one comparison of radiation dose and pathological findings.2015

    • Author(s)
      Okumura Y, Aikou S, Onoyama H, Jinbo K, Yamagata Y, Mori K, Yamashita H, Nomura S, Takahashi M, Koyama K, Momose T, Abe H, Matsusaka K, Ushiku T, Fukayama M, Seto Y.
    • Journal Title

      World J Surg Oncol.

      Volume: 13 Issue: 1 Pages: 327-327

    • DOI

      10.1186/s12957-015-0743-y

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pulmonary Metastasis of Combined Hepatocellular and Cholangiocarcinoma: A Unique Radiographic Presentation with Air-space Consolidation Masquerading as Pneumonia and Primary Pulmonary Adenocarcinoma2015

    • Author(s)
      3.Ishii T, Goto Y, Matsuzaki H, Ohishi N, Sakamoto Y, Saito R, Matsusaka K, Shibahara J, Nagase T.
    • Journal Title

      Internal Medicine

      Volume: 54 Issue: 11 Pages: 1389-1392

    • DOI

      10.2169/internalmedicine.54.3375

    • NAID

      130005072929

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA methylation in gastric cancer, related to Helicobacter pylori and Epstein-Barr virus2014

    • Author(s)
      Keisuke Matsusaka, Sayaka Funata, Masashi Fukayama and Atsushi Kaneda.
    • Journal Title

      World Journal of Gastroenterology

      Volume: 20(14) Issue: 14 Pages: 3916-3926

    • DOI

      10.3748/wjg.v20.i14.3916

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of histone demethylase KDM6B enhances aggressiveness of pancreatic cancer through downregulation of C/EBPα2014

    • Author(s)
      Yamamoto K, Tateishi K, Kudo Y, Sato T, Yamamoto S, Miyabayashi K, Asaoka Y, Ijichi H, Hirata Y, Otsuka M, Nakai Y, Isayama H, Ikenoue T, Kurokawa M, Fukayama M, Kokudo N, Omata M and Koike K.
    • Journal Title

      Carcinogenesis

      Volume: 35 Issue: 11 Pages: 2404

    • DOI

      10.1093/carcin/bgu136

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylation epigenotypes and genetic features in colorectal laterally spreading tumors2014

    • Author(s)
      Sakai E, Ohata K, Chiba H, Matsuhashi N, Doi N, Fukushima J, Endo H, Takahashi H, Tsuji S, Yagi K, Matsusaka K, Aburatani H, Nakajima A, Kaneda A.
    • Journal Title

      Int J Cancer

      Volume: in press Issue: 7 Pages: 1586-95

    • DOI

      10.1002/ijc.28814

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An indeterminate result of QuantiFERON-TB Gold In-Tube for miliary tuberculosis due to a high level of IFN-γ production2014

    • Author(s)
      Sho Hangai, Akihide Yoshimi, Atsuko Hosoi, Keisuke Matsusaka, Motoshi Ichikawa, Masashi Fukayama, Mineo Kurokawa
    • Journal Title

      International Journal of Hematology

      Volume: 99 Issue: 4 Pages: 523-526

    • DOI

      10.1007/s12185-014-1504-3

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Genetic aberration analysis combined with DNA methylome characterization in gastric cancer2015

    • Author(s)
      Keisuke Matsusaka, Masaki Fukuyo, Masayuki Urabe, Bahityar Rahmutulla, Eiji Sakai, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda
    • Organizer
      74th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      愛知県名古屋市名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Epstein-Barr virus infection induces de novo DNA methylation in non-cancerous gastric epithelial cells2014

    • Author(s)
      Keisuke Matsusaka, Sayaka Funata, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda
    • Organizer
      73rd Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama(神奈川県横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] デジタルPCRを用いた胃癌におけるHER2遺伝子の定量的解析2014

    • Author(s)
      松坂恵介,石川俊平,金子伸行,牛久哲男,深山正久
    • Organizer
      第103回 日本病理学会 総会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Remarks] 千葉大学分子腫瘍学

    • URL

      http://www.m.chiba-u.ac.jp/class/moloncol/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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