Clarification of mechanism in induction of DNA methylation using Epstein-Barr virus infection experiment

• Project/Area Number: 26860257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Chiba University
• Principal Investigator: Matsusaka Keisuke 千葉大学, 医学(系)研究科(研究院), 助教 (40610150)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エピジェネティクス / 胃癌 / Epstein-Barr virus

Outline of Final Research Achievements

Genome-wide DNA methylation analysis in early phase of Epstein-Barr virus infection revealed how aberrant DNA methylation could be induced around transcription start sites (TSSs) or in genebody and intergenic regions. DNA methylation occurred from the CpG shore or shelves to CpG islands. In expression analysis by RNA-sequencing, dynamic expression alteration of both host cells and EBV could be observed. These findings should be significant clues to elucidate the mechanisms of induction of aberrant DNA methylation.

Research Products

• [Journal Article] Genetic and epigenetic aberrations occurring in colorectal tumors associated with serrated pathway (2016)
  • Author(s): Sakai E, Fukuyo M, Ohata K, Matsusaka K, Doi N, Mano Y, Takane K, Abe H, Yagi K, Matsuhashi N, Fukushima J, Fukayama M, Akagi K, Aburatani H, Nakajima A, Kaneda A.
  • Journal Title: International Journal of Cancer Volume: 138 Pages: 1634-1644
  • DOI: 10.1002/ijc.29903
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of (18)F-FDG uptake for detecting lymph node metastasis of gastric cancer: a prospective pilot study for one-to-one comparison of radiation dose and pathological findings. (2015)
  • Author(s): Okumura Y, Aikou S, Onoyama H, Jinbo K, Yamagata Y, Mori K, Yamashita H, Nomura S, Takahashi M, Koyama K, Momose T, Abe H, Matsusaka K, Ushiku T, Fukayama M, Seto Y.
  • Journal Title: World Journal of Surgical Oncology Volume: 13 Pages: 327-327
  • DOI: 10.1186/s12957-015-0743-y
  • Peer Reviewed / Open Access
• [Journal Article] Pulmonary Metastasis of Combined Hepatocellular and Cholangiocarcinoma: A Unique Radiographic Presentation with Air-space Consolidation Masquerading as Pneumonia and Primary Pulmonary Adenocarcinoma (2015)
  • Author(s): 3.Ishii T, Goto Y, Matsuzaki H, Ohishi N, Sakamoto Y, Saito R, Matsusaka K, Shibahara J, Nagase T.
  • Journal Title: Intern. Med. Volume: 54(11) Issue: 11 Pages: 1389-1392
  • DOI: 10.2169/internalmedicine.54.3375
  • NAID: 130005072929
  • ISSN: 0918-2918
  • Peer Reviewed / Open Access
• [Journal Article] DNA methylation in gastric cancer, related to Helicobacter pylori and Epstein-Barr virus (2014)
  • Author(s): ①Matsusaka K, Funata S, Fukayama M, Kaneda A
  • Journal Title: World J Gastroenterol Volume: 20(14) Pages: 3916-26
  • DOI: 10.3748/wjg.v20.i14.3916
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loss of histone demethylase KDM6B enhances aggressiveness of pancreatic cancer through downregulation of C/EBPα (2014)
  • Author(s): Yamamoto K, Tateishi K, Kudo Y, Sato T, Yamamoto S, Miyabayashi K, Asaoka Y, Ijichi H, Hirata Y, Otsuka M, Nakai Y, Isayama H, Ikenoue T, Kurokawa M, Fukayama M, Kokudo N, Omata M and Koike K.
  • Journal Title: Carcinogenesis. Volume: 35 Pages: 2404-14
  • DOI: 10.1093/carcin/bgu136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Methylation epigenotypes and genetic features in colorectal laterally spreading tumors. (2014)
  • Author(s): Sakai E, Ohata K, Chiba H, Matsuhashi N, Doi N, Fukushima J, Endo H, Takahashi H, Tsuji S, Yagi K, Matsusaka K, Aburatani H, Nakajima A, Kaneda A.
  • Journal Title: International Journal of Cancer Volume: in press Pages: 1586-1595
  • DOI: 10.1002/ijc.28814
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] An indeterminate result of QuantiFERON-TB Gold In-Tube for miliary tuberculosis due to a high level of IFN-γ production (2014)
  • Author(s): Sho Hangai, Akihide Yoshimi, Atsuko Hosoi, Keisuke Matsusaka, Motoshi Ichikawa, Masashi Fukayama, Mineo Kurokawa
  • Journal Title: International Journal of Hematology Volume: 99 Pages: 523-526
  • DOI: 10.1007/s12185-014-1504-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Genetic aberration analysis combined with DNA methylome characterization in gastric cancer (2015)
  • Author(s): Keisuke Matsusaka, Masaki Fukuyo, Masayuki Urabe, Bahityar Rahmutulla, Eiji Sakai, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda
  • Organizer: 74th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 愛知県名古屋市名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] Epstein-Barr virus infection induces de novo DNA methylation in non-cancerous gastric epithelial cells (2014)
  • Author(s): Keisuke Matsusaka, Sayaka Funata, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda
  • Organizer: 73rd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Pacifico Yokohama（神奈川県横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] デジタルPCRを用いた胃癌におけるHER2遺伝子の定量的解析 (2014)
  • Author(s): 松坂恵介，石川俊平，金子伸行，牛久哲男，深山正久
  • Organizer: 第103回 日本病理学会 総会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Remarks] 千葉大学分子腫瘍学
  • URL: http://www.m.chiba-u.ac.jp/class/moloncol/