Elucidation of role and function for cell polarity in Hair follicle stem cells through hemidesmosomal components

• Project/Area Number: 26860258
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Matsumura HIroyuki 東京医科歯科大学, 難治疾患研究所, 助教 (70581700)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 組織幹細胞 / 幹細胞 / 細胞極性 / ヘミデスモソーム / COL17A1 / ITGA6

Outline of Final Research Achievements

Cell polarity plays important functional roles in differentiation and proliferation of mammalian stem cells. However, the function and regulation of cell polarity in hair follicle stem cells (HFSCs) are unclear. COL17A1 is one of the hemidesmosomal transmembrane proteins that play a critical role in maintaining the linkage between the intracellular and the extracellular structural elements involved in epidermal integrity. Previously, we showed that COL17A1 is essential for maintenance of hair follicle stem cells (Cell Stem Cell 2011), that the maintenance of COL17A1 in HFSCs orchestrates the stem cell centric aging program of the epithelial mini-organ (Matsumura et al. Science 2016). In this study, we are investigating how COL17A1 and ITGA6 regulates the fate of hair follicle stem cells by focusing on cell polarity.

Research Products

• [Journal Article] Hair follicle aging is driven by transepidermal stem cell elimination via COL17A1 proteolysis. (2016)
  • Author(s): Matsumura H, Mohri Y, Binh N T, Morinaga H, Fukuda M, Ito M, Kurata S, Hoeijmakers J, Nishimura E K
  • Journal Title: Science Volume: 351 Pages: 6273-6273
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Calreticulin is required for development of the cumulus oocyte complex and female fertility. (2015)
  • Author(s): Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham AM, Ikawa M.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 14254-14254
  • DOI: 10.1038/srep14254
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] メラノーマの発生と色素幹細胞の運命制御 (2015)
  • Author(s): 西村 栄美、毛利 泰彰、Sally, Eshiba, Leu, Nan, 松村 寛行
  • Organizer: 第７４回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋）
  • Year and Date: 2015-10-08
• [Presentation] 老化・発癌ストレス下における毛包幹細胞のクローン運命解析 (2015)
  • Author(s): 高田亜希, 松村寛行, 岡崎 睦, 西村栄美.
  • Organizer: 第24回日本形成外科学会基礎学術集会
  • Place of Presentation: 岩手県民会館（岩手県・盛岡市）
  • Year and Date: 2015-10-08
• [Presentation] Defective maintenance of COL17A1 in hair follicle stem cells orchestrates hair follicle aging (2014)
  • Author(s): Hiroyuki Matsumura, Mohri Yasuaki, Nguyen Thanh Binh, Morinaga Hironobu, Emi K Nishimura
  • Organizer: 12th Stem Cell Research Symposium
  • Place of Presentation: fukuoka
  • Year and Date: 2014-05-30 – 2014-05-31
• [Patent(Industrial Property Rights)] 脱毛および白毛化を抑制もしくは改善するための組成物ならびにその使用 (2016)
  • Inventor(s): 西村 栄美、 松村 寛行
  • Industrial Property Rights Holder: 西村 栄美、 松村 寛行
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-003424
  • Filing Date: 2016-01-12