Establishment of model for lung adenocarcinoma in 3D and identification of promotional factor for stromal invasion
| Project/Area Number |
26860268
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Kinki University |
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Principal Investigator |
KATO Takashi 近畿大学, 医学部, 助教 (40573423)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | HGF / 肺胞上皮細胞 / 三次元培養 / 非浸潤 / ３次元培養 / 肺腺癌 |
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| Outline of Final Research Achievements |
Bronchial epithelial cells could differentiate into multiple lung cell type and develop multiple foci with well-differentiated histogenesis after transformed into neoplastic cells. We investigated morphogenic abilities of HBE135, immortalized human bronchial epithelial cells, in 3D cultures. In culture with lung fibroblast MRC-9 cells, HBE135 cells formed colonies with bronchioalveolar-like complex branching. MRC-9 cells highly express HGF, and an anti-HGF neutralizing antibody suppressed the branching formation. But HGF could not sufficiently compensate the morphogenic effects of MRC-9 cells, suggesting that HGF was necessary but insufficient for the branching formation. Furthermore, Met was phosphorylated in neoplastic bronchial epithelial cells from lung well-differentiated adenocarcinomas. Besides, Met was strongly expressed in BASCs, distal lung epithelial stem cells. In alveoli, we found higher expression of Met in primary alveolar type 2 (AT2) than in AT1 cells.
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Report
(3 results)
Research Products
(11 results)
