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Establishment of model for lung adenocarcinoma in 3D and identification of promotional factor for stromal invasion

Research Project

Project/Area Number 26860268
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionKinki University

Principal Investigator

KATO Takashi  近畿大学, 医学部, 助教 (40573423)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsHGF / 肺胞上皮細胞 / 三次元培養 / 非浸潤 / 3次元培養 / 肺腺癌
Outline of Final Research Achievements

Bronchial epithelial cells could differentiate into multiple lung cell type and develop multiple foci with well-differentiated histogenesis after transformed into neoplastic cells. We investigated morphogenic abilities of HBE135, immortalized human bronchial epithelial cells, in 3D cultures. In culture with lung fibroblast MRC-9 cells, HBE135 cells formed colonies with bronchioalveolar-like complex branching. MRC-9 cells highly express HGF, and an anti-HGF neutralizing antibody suppressed the branching formation. But HGF could not sufficiently compensate the morphogenic effects of MRC-9 cells, suggesting that HGF was necessary but insufficient for the branching formation. Furthermore, Met was phosphorylated in neoplastic bronchial epithelial cells from lung well-differentiated adenocarcinomas. Besides, Met was strongly expressed in BASCs, distal lung epithelial stem cells. In alveoli, we found higher expression of Met in primary alveolar type 2 (AT2) than in AT1 cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 6 results) Presentation (4 results)

  • [Journal Article] Decreased expression of Met during differentiation in rat lung2016

    • Author(s)
      T Kato, K Oka, T Nakamura, A Ito
    • Journal Title

      European Journal of Histochemistry

      Volume: 60 Issue: 1 Pages: 2575-2575

    • DOI

      10.4081/ejh.2016.2575

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bronchioalveolar morphogeneis of human bronchial epithelial cells depending upon hepatocyte growth factor.2015

    • Author(s)
      Kato T, Oka K, Nakamura T, Ito A
    • Journal Title

      J Cell Mol Med

      Volume: 19 Issue: 12 Pages: 2818-2826

    • DOI

      10.1111/jcmm.12672

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Blood pressure, renal biochemical parameters and histopathology in a original rat model of essential hypertension (SHRSP/Kpo strain)2015

    • Author(s)
      Kato T, Mizguchi N, Ito A
    • Journal Title

      Biomedical Research

      Volume: 印刷中

    • NAID

      130005083861

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Stromal cell-derived factor-1 (SDF1)-dependent recruitment of bone marrow-derived renal endothelium-like cells in a mouse model of acute kidney injury2015

    • Author(s)
      Ohnishi H, Mizuno S, Mizuno-Horikawa Y and Kato T.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 3 Pages: 313-319

    • DOI

      10.1292/jvms.14-0562

    • NAID

      130005060999

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of core active disaccharides in heparin for HGF-inducing activity.2015

    • Author(s)
      Kato T, Sakiyama R, Oka K, Nakamura T.
    • Journal Title

      Journal of Pharamacology Pharmacoltherpeutics

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Decrease in Glomerular Endothelial Cells and Endothelial-mesenchymal Transition during Glomerulosclerosis in the <i>Tensin2</i>-deficient Mice (ICGN strain)2014

    • Author(s)
      Kato T, Mizuno S and Ito A
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 47 Issue: 6 Pages: 265-271

    • DOI

      10.1267/ahc.14032

    • NAID

      130004703745

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Increased ectodomain shedding of cell adhesion molecule 1 from pancreatic islets in type 2 diabetic pancreata: correlation with hemoglobin A1c levels.2014

    • Author(s)
      Inoue T, et al
    • Journal Title

      Plos One

      Volume: 9 Issue: 6 Pages: 00-00

    • DOI

      10.1371/journal.pone.0100988

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 肺胞再生の分子機構解明に向けた3次元培養モデルを構築する2015

    • Author(s)
      加藤貴史、伊藤彰彦、高橋英夫
    • Organizer
      日本薬理学会関東部会
    • Place of Presentation
      千葉 明海大学
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] HGF accelerates the serine44-phosphorylation and cell surface translocation of ROMK channel in rat kidney cells2015

    • Author(s)
      加藤 貴史、伊藤彰彦
    • Organizer
      日本薬理学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Research-status Report
  • [Presentation] HGF誘導活性に関与するへパリン2糖の硫酸基の同定2014

    • Author(s)
      加藤 貴史、崎山 亮一、岡 清正、伊藤彰彦、中村 敏一
    • Organizer
      日本生化学会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Lung adenomatous tumor-like construction in three-dimensional culture2014

    • Author(s)
      加藤 貴史、岡 清正、中村敏一、伊藤彰彦
    • Organizer
      日本癌学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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