| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
JC virus is a DNA virus causing progressive multifocal leukoencephalopathy (PML). We identified a JC virus quasi-species with an amino acid substitution in the large T antigen in patients with PML by next-generation sequencing. In vitro studies showed that the mutation strongly repressed the expression of viral proteins and reduced the viral replication. However, immunohistochemistry revealed that the expression of viral proteins was sustained in vivo. Thus, JC virus replicates in PML lesions in the presence of a mutant virus which is able to suppress virus replication.
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