| Project/Area Number |
26860272
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | National Center for Global Health and Medicine |
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Principal Investigator |
Yabe Shigeharu 国立研究開発法人国立国際医療研究センター, その他部局等, 研究員 (40533716)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 糖尿病 / 膵β細胞 / iPS細胞 / 疾患iPS / MODY |
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| Outline of Final Research Achievements |
We established a six-stage protocol mimicking the developmental processes of pancreatic beta cells for the differentiation of human iPSC to pancreatic beta cells using defined culture media without feeders or serum . Spheroid formation at the pancreatic beta cells stage showed more efficient insulin secretion than did monolyater culture. Cultured cells were transpranted under kidney capsules of streptozotocin-induced diabetic NOD/SCID mice. After cell trans plantation, diabetic mice had lower blood glucose levels, and islet-like struture were detected in vivo. Moreover, 12 weeks after transplantation, Human C-peptide was detected in the mouse serum .Tthese results indicated that iPS-beta cells fanctioned in vio.
We also established the iPSC from Maturity-onset diabetes of the young (MODY) 5 patient. In the course of differentiation from MODY5-iPS cells into pancratic beta cells, we found that the amount of mutant transcripts was much less than that of wild one.
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