Mechanism of protective immunity against malaria parasite parasitized erhtroblasts

• Project/Area Number: 26860276
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Gunma University
• Principal Investigator: Imai Takashi 群馬大学, 医学(系)研究科(研究院), 助教 (10513434)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: マラリア / 赤芽球 / CD8T細胞 / マクロファージ / 免疫 / Fas / フォスファチジルセリン / Plasmodium / CD８T細胞

Outline of Final Research Achievements

We recently discovered that mouse malaria parasites can parsitize erythroblasts which is the precursor of the RBC.Parasitized erythroblast express MHC class I and CD8 T cells recognized them an antigen-specific manner.
In this study, we found that CD8 T cells are important to protection against parasitized eryhthroblast. FasL on CD8 T cells interacts with Fas (death receptor) on the parasitized erythroblasts and induces externalization of phosphatidylserine (PS).　PS externalized infected cell are phagocytized by macrophage though Tim-4.

Research Products

• [Journal Article] A transient resistance to blood-stage malaria in interferon-γ-deficient mice through impaired production of the host cells preferred by malaria parasites. (2015)
  • Author(s): Okada H, Suzue K, Imai T, Taniguchi T, Shimokawa C, Onishi R, Hirata J, Hisaeda H.
  • Journal Title: Front Microbiol. Volume: 6 Pages: 600-600
  • DOI: 10.3389/fmicb.2015.00600
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis (2015)
  • Author(s): Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T, Hisaeda H
  • Journal Title: Scientific Reports Volume: 5:15699 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep15699
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cytotoxic activities of CD8+ T cells collaborate with macrophages to protect against blood-stage murine malaria (2015)
  • Author(s): Imai T, Ishida H, Suzue K, Taniguchi T, Okada H, Shimokawa C, and Hisaeda H
  • Journal Title: eLIFE Volume: 4 Pages: 4-4
  • DOI: 10.7554/elife.04232
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluating experimental cerebral malaria using oxidative stress indicator OKD48 mice. (2014)
  • Author(s): Imai T, Iwawaki T, Akai R, Suzue K, Hirai M, Taniguchi T, Okada H, Hisaeda H.
  • Journal Title: Int J Parasitol. Volume: 44 Issue: 10 Pages: 681-685
  • DOI: 10.1016/j.ijpara.2014.06.002
  • Peer Reviewed / Open Access
• [Presentation] Cytotoxic activities of CD8+ T cells collaborate with macrophages to protect against blood-stage murine malaria (2014)
  • Author(s): Takashi Imai; Hidekazu Ishida; Kazutomo Suzue; Makoto Hirai; Tomoyo Taniguchi; Hiroko Okada1; Chikako Shimokawa3; and Hajime Hisaeda1
  • Organizer: International congress of parasitology XIII
  • Place of Presentation: メキシコシティ
  • Year and Date: 2014-08-10 – 2014-08-15
• [Remarks] 群馬大学プレスリリース
  • URL: http://www.gunma-u.ac.jp/sb/sb.cgi?eid=1086