• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mutation patterns of mitochondrial DNA in atovaquone-resistant malaria parasites

Research Project

Project/Area Number 26860277
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Parasitology (including sanitary zoology)
Research InstitutionChiba University (2015)
Teikyo University (2014)

Principal Investigator

Hikosaka Kenji  千葉大学, 医学(系)研究科(研究院), 特任助教 (30456933)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsマラリア / Plasmodium / 薬剤耐性 / 抗マラリア薬 / アトバコン / ミトコンドリアゲノム / 動物感染モデル
Outline of Final Research Achievements

To clarify mechanism of emergence of resistance to antimalarial drugs, we investigated emergence patterns of a point mutation associated with drug resistance using Plasmodium berghei-infected mice. In this study, atovaquone (ATQ), targeting cytochrome b (cob) gene encoded on mitochondrial DNA (mtDNA) of Plasmodium, was selected as an antimalarial drug. In a result, four non-synonymous mutations were found in cob; three mutations (L271V、K272R and V284F) were already reported and the remaining one (I258M) was new. In order to further elucidate emergence patterns of these point mutations, we performed deep sequencing of Plasmodium mtDNA, which was extracted from infected blood collected continuously, using a next-generation sequencer. Analysis of the deep sequencing revealed that the mutation (V284F) was dramatically emerged six days after administration of ATQ. In the future, we will investigate emergence pattern of the other mutations (L271V、K272R and I258M) by deep sequencing.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Mitochondrial genome structure of the phylum Apicomplexa2015

    • Author(s)
      Hikosaka K, Tanabe K, Kita K
    • Journal Title

      Jpn J Protozool

      Volume: 48 Pages: 45-56

    • NAID

      130005254780

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lactate retards the development of erythrocytic stages of the human malaria parasite Plasmodium falciparum.2015

    • Author(s)
      Hikosaka K, Hirai M, Komatsuya K, Ono Y, Kita K.
    • Journal Title

      Parasitol Int

      Volume: 64 Issue: 3 Pages: 301-303

    • DOI

      10.1016/j.parint.2014.08.003

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マラリア原虫の遺伝子点変異によるアトバコン耐性獲得様式の解明2016

    • Author(s)
      彦坂健児、本間一、松崎素道、野呂瀬一美、北潔
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県・宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] マラリア原虫の遺伝子点変異による薬剤耐性獲得様式の解明2015

    • Author(s)
      彦坂健児、本間一、松崎素道、野呂瀬一美、北潔
    • Organizer
      第23回分子寄生虫ワークショップ/第13回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学・原虫病研究センター PKホール(北海道・帯広市)
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] アピコンプレクサ原虫の多様なミトコンドリアゲノム構造2014

    • Author(s)
      彦坂健児
    • Organizer
      第47回日本原生生物学会大会
    • Place of Presentation
      宮城
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] An Overview of Tropical Diseases, Chapter 2, Mitochondria of malaria parasites as a drug target2015

    • Author(s)
      Hikosaka K, Komatsuya K, Suzuki S, Kita K
    • Publisher
      Intech
    • Related Report
      2015 Annual Research Report
  • [Book] An Overview of Tropical Diseases -Mitochondria of Malaria Parasites as A Drug Target2015

    • Author(s)
      Hikosaka K, Komatsuya K, Suzuki S, Kita K
    • Total Pages
      18
    • Publisher
      Intech
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi