Genome-wide immunoscreening of antibodies responsible for Plasmodium falciparum growth inhibitory activity of Malian adult IgG

• Project/Area Number: 26860279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: Morita Masayuki 愛媛大学, プロテオサイエンスセンター, 研究員 (60709632)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マラリアワクチン候補抗原 / コムギ胚芽無細胞タンパク質合成法 / GIA / ハイスループットスクリーニング

Outline of Final Research Achievements

To identify novel malaria vaccine candidates, I used 1900 Plasmodium falciparum proteins prepared using wheat germ cell-free system. Using the proteins, I measured the immunoreactivity of Malian adult IgG samples. Antibody reactivities against 3 proteins were positively correlated with growth inhibitory activity against in vitro cultured P.falciparum. Immunoreactivity of LSA3 has the highest correlation coefficient. Anti LSA3 specific antibody purified from Malian adult IgGs showed growth inhibitory activity against P.falciparum. The results suggest that LSA3 is a blood-stage malaria vaccine candidate.

Research Products

• [Journal Article] Vaccine candidates for malaria: what's new? (2016)
  • Author(s): Takashima E., Morita M., Tsuboi T.
  • Journal Title: Expert Review of Vaccines Volume: 15 Issue: 1 Pages: 1-3
  • DOI: 10.1586/14760584.2016.1112744
  • Peer Reviewed
• [Journal Article] Stage specific activity of synthetic antimalarial endoperoxides, N-89 and N-251, against Plasmodium falciparum. (2015)
  • Author(s): Morita, M., Koyama, T., Sanai, H., Sato, K., hiramoto, A., Masuyama, A., Nojima, M., Wataya, Y. and Kim, H.-S
  • Journal Title: Parasitol. Int. Volume: 64 Issue: 1 Pages: 113-117
  • DOI: 10.1016/j.parint.2014.10.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] タンパク質輸送機構に関連すると考えられる熱帯熱マラリア原虫デンスグラニュールタンパク質PV1の機能解析 (2016)
  • Author(s): 森田将之、高島英造、長岡ひかる、伊藤大輔、Bernard N. Kanoi、中田貴敬、西莉菜、飯村忠浩、鳥居本美、坪井敬文
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19
• [Presentation] Genome-wide immunoscreening of antibodies responsible for Plasmodium falciparum growth inhibitory activity of Malian adult IgG (2015)
  • Author(s): Morita M., Takashima E., Kanoi B.N., Miura K., Long C.A., Tsuboi T.
  • Organizer: The American Society of Tropical Medicine and Hygiene (ASTMH) 64th Annual Meeting
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] Genome-wide immunoscreening of antigens contributing to Plasmodium falciparum growth inhibitory activity of Malian adult IgG (2015)
  • Author(s): Morita M., Takashima E., Kanoi B.N., Miura K., Long C.A., Tsuboi T.
  • Organizer: Protein Island Matsuyama (PIM) International Symposium 2015, The 13th Matsuyama International Symposium on Proteo-Sciences
  • Place of Presentation: Matsuyama (Japan)
  • Year and Date: 2015-09-25
  • Int'l Joint Research
• [Presentation] 超解像顕微鏡によるマラリア原虫デンスグラニュールタンパク質群の寄生胞内動態解析 (2015)
  • Author(s): 森田将之、高島英造、伊藤大輔、中田貴敬、Bernard N. Kanoi、飯村忠浩、鳥居本美、坪井敬文
  • Organizer: 第２３回分子寄生虫学ワークショップ／ 第１３回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学・原虫病研究センター PKホール（北海道帯広市）
  • Year and Date: 2015-08-30
• [Presentation] Genome-wide immunoscreening to identify blood-stage malaria vaccine candidates of Plasmodium falciparum (2015)
  • Author(s): Masayuki Morita, Eizo Takashima, Bernard N. Kanoi, Kazutoyo Miura, Carole A. Long, Takafumi Tsuboi
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: UGA Hotel and Conference Center, Athens, GA USA
  • Year and Date: 2015-04-28 – 2015-04-29
• [Presentation] Genome-wide immunoscreening to identify blood-stage malaria vaccine candidates of Plasmodium falciparum (2015)
  • Author(s): Morita M., Takashima E., Kanoi B.N., Miura K., Long C.A., Tsuboi T.
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: Athens (USA)
  • Year and Date: 2015-04-28
  • Int'l Joint Research
• [Presentation] 免疫スクリーニングによる熱帯熱マラリア原虫ゲノム網羅的な赤血球期ワクチン候補抗原の探索 (2015)
  • Author(s): 森田将之、高島英造、Bernard N. Kanoi、三浦憲豊、坪井敬文
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Immunoscreening of the target proteins contributing to growth inhibitory activity of human IgG against Plasmodium falciparum (2014)
  • Author(s): Masayuki Morita, Eizo Takashima, Bernard N. Kanoi, Kazutoyo Miura, Takafumi Tsuboi
  • Organizer: The American Society of Tropical Medicine and Hygiene (ASTMH) 63rd Annual Meeting
  • Place of Presentation: Sheraton New Orleans Hotel and New Orleans Marriott Hotel, New Orleans, LA USA
  • Year and Date: 2014-11-02 – 2014-11-06
• [Presentation] Genome-wide screening of the antigens contributing to growth inhibitory activity of human IgG against Plasmodium falciparum in Malian adults (2014)
  • Author(s): Masayuki Morita, Eizo Takashima, Bernard N. Kanoi, Kazutoyo Miura, Takafumi Tsuboi
  • Organizer: Protein Island Matsuyama (PIM) International Symposium 2014, The 12th Matsuyama International Symposium on Proteo-Sciences
  • Place of Presentation: 愛媛大学
  • Year and Date: 2014-09-17
• [Presentation] 原虫増殖阻害活性と抗体価の相関解析によるマラリアゲノム網羅的ワクチン候補探索 (2014)
  • Author(s): 森田将之、高島英造、Bernard N. Kanoi、三浦憲豊、坪井敬文
  • Organizer: 第22回分子寄生虫学ワークショップ／第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学原虫病研究センターPKホール
  • Year and Date: 2014-08-31 – 2014-09-03