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Tempeature-dependent attachment regulation via type IV pili in Clostridia

Research Project

Project/Area Number 26860281
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionUniversity of Tsukuba

Principal Investigator

OBANA Nozomu  筑波大学, 生命環境系, 助教 (00722688)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsクロストリジウム属細菌 / バイオフィルム / IV型線毛 / 温度変化 / ウェルシュ菌 / IV線毛 / 付着 / RNA切断
Outline of Final Research Achievements

Clostridium perfringens survives antibiotic treatment or oxidative stress through the formation of biofilms or spores, but the environmental and regulatory factors involved in the biofilm formation remain unclear. Here, we observed that temperature regulates C. perfringens biofilm morphology. At 37°C, C. perfringens adhered to the substrate surface and formed a flat, thin biofilm. However, at 25°C, this bacterium did not adhere and produced a threadlike extracellular matrix, forming a viscous, thick biofilm. Adhered-biofilm formation requires pilA2, which encodes a component of type IV pili (TFP). TFP expression was activated at 37°C. These results indicate that the morphology of C. perfringens biofilm is dependent on temperature through the differential production of extracellular matrix and the activity of TFP. Here, we demonstrated that the morphological change of the biofilms via TFP activity could play an important role in the pathogenesis of this organism

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Environmental factors that shape biofilm formation.2015

    • Author(s)
      Toyofuku M, Inaba T, Kiyokawa T, Obana N, Yawata Y, Nomura N.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 1 Pages: 7-12

    • DOI

      10.1080/09168451.2015.1058701

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 集団が生み出す新たな微生物挙動~集団微生物学の勧め2015

    • Author(s)
      稲葉知大、尾花望、清川達則、吉田圭太朗、佐々文洋、尾形敦、野村暢彦
    • Journal Title

      ソフトドリンク技術資料

      Volume: 177 Pages: 411-433

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A sporulation factor is involved in the morphological change of Clostridium perfringens biofilms in response to temperature.2014

    • Author(s)
      Obana N, Nakamura K, Nomura N.
    • Journal Title

      Journal of Bacteriology

      Volume: 196 Issue: 8 Pages: 1540-1550

    • DOI

      10.1128/jb.01444-13

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A novel approach for purification and selective capture of membrane vesicles of the periodontopathic bacterium, Porphyromonas gingivalis: membrane vesicles bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner.2014

    • Author(s)
      Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 5 Pages: 1-10

    • DOI

      10.1371/journal.pone.0095137

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 集団微生物学のすすめ~バイオフィルムとその解析技術2014

    • Author(s)
      稲葉知大、清川達則、尾花 望、豊福雅典、八幡 穣、野村暢彦
    • Journal Title

      化学と生物: 日本農芸化学会会誌

      Volume: 52 Pages: 594-601

    • NAID

      130005096043

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Immunoactive membrane vesicles are actively produced by the Gram-positive clostridial pathogen2016

    • Author(s)
      尾花 望、中尾龍馬、永山恭子、泉福英信、野村暢彦
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター 大阪府大阪市
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] グラム陽性細菌が能動的につくる膜小胞体(メンブランベシクル)と宿主への作用2015

    • Author(s)
      尾花望
    • Organizer
      日本乳酸菌学会2015年度秋期セミナー
    • Place of Presentation
      昭和女子大学 東京都 世田谷区
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] An extracellular self-assembling protein coating the Clostridium perfringens biofilm2015

    • Author(s)
      Nozomu Obana, Kouji Nakamura, Nobuhiko Nomura
    • Organizer
      7th ASM Conference on Biofilms
    • Place of Presentation
      Chicago, IL, US
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウェルシュ菌における外界の環境に応答したバイオフィルム形態制御2015

    • Author(s)
      ○ 尾花 望、中村幸治、野村暢彦
    • Organizer
      第29回日本バイオフィルム学会
    • Place of Presentation
      ホテル竹島 愛知県蒲郡市
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] グラム陽性細菌のメンブランベシクルと動物細胞への作用2015

    • Author(s)
      尾花 望、中尾龍馬、中村幸治、泉福英信、野村暢彦
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ウェルシュ菌バイオフィルム構造形成に必須な細胞外マトリクスタンパク質2015

    • Author(s)
      尾花 望、中村幸治、野村暢彦
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] ウェルシュ菌バイオフィルムマトリクスオペロンの同定2015

    • Author(s)
      尾花 望、中村幸治、野村暢彦
    • Organizer
      第9回ゲノム微生物学会年会
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 芽胞形成細菌における環境中の温度に応答したバイオフィルム形態変化2014

    • Author(s)
      尾花 望、中村幸治、野村暢彦
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松コングレスセンター(静岡県浜松市)
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 外界の温度に対するウェルシュ菌のバイオフィルム形態変化2014

    • Author(s)
      尾花 望、中村幸治、野村暢彦
    • Organizer
      日本農芸化学会関東支部2014年度支部大会
    • Place of Presentation
      埼玉大学(埼玉県さいたま市)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] A sporulation factor and multiple orphan kinases control the morphological changes of Clostridium perfringens biofilms in response to temperature2014

    • Author(s)
      Nozomu Obana, Kouji Nakamura, Nobuhiko Nomura
    • Organizer
      ASM general meeting 2014
    • Place of Presentation
      Boston, America
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Research-status Report
  • [Book] 月刊バイオインダストリー 2016年3月号 微生物間相互作用を利用した複合系バイオフィルムの制御2016

    • Author(s)
      豊福雅典、尾花望、野村暢彦
    • Total Pages
      5
    • Publisher
      シーエムシー
    • Related Report
      2015 Annual Research Report
  • [Remarks] 野村暢彦研究室

    • URL

      http://www.envr.tsukuba.ac.jp/~microbio/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 筑波大学 野村暢彦研究室

    • URL

      http://www.envr.tsukuba.ac.jp/~microbio/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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