Analysis of a novel virulence factor produced by Bordetella bronchiseptica
| Project/Area Number |
26860288
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAMURA Keiji 大阪大学, 微生物病研究所, 特任研究員(常勤) (60706216)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ボルデテラ属細菌 / 気管支敗血症菌 / バイオフィルム / 二成分制御系 / I型分泌機構 / 付着因子 |
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| Outline of Final Research Achievements |
In the present study, the gene brtA (Bordetella RTX-family Adhesin), which was identified as one of genes expressed in B. bronchiseptica infecting host respiratory tracts, was characterized. Expression of virulence factors in Bordetella is mainly controlled by a two-component regulatory BvgAS system, which regulates a bacterial phase conversion between virulent Bvg+ and avirulent Bvg- phases. Immunoblotting and qRT-PCR analysis revealed that BrtA was expressed and secreted from the bacteria in the Bvg- phase. Furthermore, qRT-PCR confirmed that brtA was expressed in the bacteria colonizing rat trachea. Phenotypic analyses of a brtA-deficient strain demonstrated that BrtA is essential for adherence to plastic plate and is involved in biofilm formation of B. bronchiseptica. Although the role of brtA during Bordetella-infection remains to be elucidated, these results imply that the genes expressed in avirulent Bvg- phase may play a certain role in the course of infection.
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Report
(3 results)
Research Products
(9 results)
