Structural analysis of type III secretion system in the immune cells
Project/Area Number |
26860289
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including mycology)
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | III型分泌装置 / サルモネラ / 電子顕微鏡 / 電子線トモグラフィー |
Outline of Final Research Achievements |
In human, infection by Salmonella enterica serovar Typhi (S. Typhi) usually causes typhoid fever. S. Typhi uses the type III secretion system (T3SS) encoded by Salmonella pathogenicity islands 2 (SPI-2) to deliver virulence effector proteins into immune cells such as dendritic cell and macrophage, thereby being able to survive and grow within host cells. However, the structure and secretion mechanism of SPI-2 T3SS are still unknown. In this study, we tried structural analysis of in situ SPI-2 T3SS in the immune cell. Unfortunately, we could not identify the structure of SPI-2 T3SS in the immune cell, but we were able to construct Salmonella mini-cell expressing enhanced green fluorescent protein (EGFP) to identify the localization of Salmonella mini-cell in the immune cells. Salmonella mini-cell with EGFP will enable the application of correlative light and electron microscopy (CLEM) to identify the structure of SPI-2 T3SS in the immune cell.
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Report
(3 results)
Research Products
(14 results)
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[Presentation] 細菌べん毛III型分泌装置のエネルギー変換メカニズム2015
Author(s)
Terashima, H., Kawamoto, A., Tatsumi, C., Minamino, T., Namba, K., Imada, K
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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[Presentation] 細菌べん毛のIII型分泌装置の再構築2015
Author(s)
寺島浩行, 川本晃大, 巽千夏, 南野徹, 難波啓一, 今田勝巳
Organizer
第88回日本細菌学会総会
Place of Presentation
岐阜県, 長良川国際会議場
Year and Date
2015-03-26 – 2015-03-28
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[Presentation] In situ structures of Salmonella injectisome and flagellar type III secretion systems by electron cryotomography2014
Author(s)
Kawamoto, A., Morimoto, Y.V., Miyata, T., Minamino, T., Hughes, K.T., Kato, T., Namba
Organizer
International Biophysics Congress
Place of Presentation
Brisbane, Australia
Year and Date
2014-08-03 – 2014-08-07
Related Report
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