| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Vibrio alginolyticus, a marine bacterium, is a causative agent of wound and ear infections. Collagenase is involved in its pathogenicity. We have previously revealed that the expression of collagenase is regulated by the VarS/VarA two-component regulatory system. In this study, we identified four small RNAs (CsrB1, CsrB2, CsrB3, CsrC) whose expressions were regulated by the VarS/VarA regulatory system. The transcriptional activities of promoters of all four small RNAs were positively regulated by the VarS/VarA regulatory system. Amount of CsrB3 was decreased in the cells of the varS and varA deletion mutant, but, inconsistent with the transcriptional activities, those of CsrB1, CsrB2 and CsrC were increased. This result suggests that there is a hitherto uncharacterized mechanism to regulate the amounts of small RNAs in the cells. In addition, we identified that HapR, the master regulator of quorum sensing system, regulates the transcriptional of collagenase.
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