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Elucidation of the mechanism of outer membrane vesicles production in Gram-negative bacteria

Research Project

Project/Area Number 26860293
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionKyoto University (2015-2016)
University of Miyazaki (2014)

Principal Investigator

Kazunori Murase  京都大学, 医学研究科, 研究員 (40710869)

Research Collaborator SHIRAISHI Tsukasa  札幌医科大学医学部, 微生物学講座, 助教
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords外膜小胞 / 国際共同研究 / HlyF / グラム陰性細菌 / 国際情報交換(フランス)
Outline of Final Research Achievements

The hemolysin F (hlyF) gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the short chain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs.
This study is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs and expected that HlyF-dependent OMV production could be one of the method for the development of vaccine.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] フランス国立保健医学研究所(INSERM)/フランス国立農学研究所(INRA)/フランス国立科学研究センター(CNRS)(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] フランス国立保健医学研究機構(INSERM)/フランス国立農学研究所(INRA)/フランス国立科学研究センター(CNRS)(フランス)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] アルマンフラピエ研究所(INRS Armand-Frappier)(カナダ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Distinct interacting core taxa in co-occurrence networks enable discrimination of polymicrobial oral diseases with similar symptoms2016

    • Author(s)
      Shiba T, Watanabe T, Kachi H, Koyanagi T, Maruyama N, Murase K, Takeuchi Y, Maruyama F, Izumi Y, Nakagawa I
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 30997-30997

    • DOI

      10.1038/srep30997

    • NAID

      120005841407

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DNA-based culture-independent analysis detects the presence of Group A Streptococcus in throat samples from healthy adults in Japan2016

    • Author(s)
      T. Kulkarni, F. Maruyama, C. Aikawa, T. Nozawa, K. Murase, I. Nakagawa.
    • Journal Title

      BMC Microbiology

      Volume: 16(1) Issue: 1 Pages: 237-237

    • DOI

      10.1186/s12866-016-0858-5

    • NAID

      120006223635

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hlyf Produced By Extraintestinal Pathogenic Escherichia Coli Is A Virulence Factor That Regulates Outer Membrane Vesicle Biogenesis2015

    • Author(s)
      Kazunori Murase, Patricia Martin, Gaëlle Porcheron, Sébastien Houle, Emmanuelle Helloin, Marie Pénary, Jean-Philippe Nougayrède, Charles M. Dozois, Tetsuya Hayashi, and Eric Oswald
    • Journal Title

      Journal of Infectious Diseases

      Volume: 213(5) Issue: 5 Pages: 856-865

    • DOI

      10.1093/infdis/jiv506

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] New mechanisms of outer membrane vesicles in Gram-negative bacteria2016

    • Author(s)
      Kazunori Murase
    • Organizer
      The International Seminar on Applied Microbial Ecology
    • Place of Presentation
      Temuco, Chile
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] HlyFによって誘導される外膜小胞の産生機構の解明2016

    • Author(s)
      村瀬一典, Patricia Martin, Jean-Philippe Nougayrede, 林哲也, Eric Oswald
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] HlyFによるOuter membrane vesicles(外膜小胞)産生誘導機構の解明2015

    • Author(s)
      村瀬 一典, Patricia Martin, Jean-Philippe Nougayrede, 林 哲也, Eric Oswald
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Role for hemolysin F in the virulence of Escherichia coli2014

    • Author(s)
      Martin P, Murase K, Houle S, Helloin E, Nougayrede JP, Dozois C, Hayashi T, Oswald E
    • Organizer
      ASM 2014 (114th General Meeting, American Society for Microbiology)
    • Place of Presentation
      Boston Convention & Exhibition Center (Boston, Massachusetts)
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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