Molecular mechanism of centrosome activation regulated by influenza virus infection
| Project/Area Number |
26860299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | インフルエンザウイルス / 中心体 / YB-1 / 核膜 / 微小管 / インフルエンザ |
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| Outline of Final Research Achievements |
YB-1 accumulates in the centrosome with influenza virus genome and formed a toroidal structure with a beads-on-a-string distribution around centriole. We also found that YB-1 localized at the centrosome in uninfected cells during mitotic phase and stimulates microtubule nucleation from the centrosome. Thus, it is likely that influenza virus recruits YB-1 at the centrosome to stimulate microtubule assembly. It is also revealed that, in infected cells, recycling endosomes accumulate in pericentrosomal organelle, called endocytic recycling compartments (ERC), through the centrosome maturation by recruiting YB-1. Further, we found that cholesterol accumulates in ERC with the virus genome and the cholesterol-enriched ERC is important for clustering of viral membrane proteins at the plasma membrane. These results suggest that cholesterol could be a trigger for budozone formation concomitantly with the virus genome trafficking to the plasma membrane (PLoS Pathog., 2015).
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
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Journal Title
Molecular cell
Volume: 53
Issue: 3
Pages: 393-406
DOI
Related Report
Peer Reviewed / Open Access
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