Characterization of HBZ specific T cells for new anti-HTLV-1 therapy
| Project/Area Number |
26860301
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
SUGATA Kenji 京都大学, ウイルス研究所, 研究員 (10650616)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | HTLV-1 / 成人T細胞白血病 / HBZ / 細胞障害性T細胞 / 遺伝子組換えワクシニアウイルス / HBZ特異的CTL / ワクチン |
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| Outline of Final Research Achievements |
HTLV bZIP factor (HBZ) is consistently expressed in HTLV-1 infected cells and ATL cells. Therefore, HBZ is a potential therapeutic target for immunotherapy of HTLV-1 related diseases and adult T cell leukemia (ATL). In this study, we generated and characterized HBZ-specific CTLs using HBZ-expressing recombinant vaccine virus (rVV-HBZ). HBZ-specific CTLs were induced in rVV-HBZ immunized mice (C57BL/6) and produced effector cytokines (IFN-γ and TNF-α) by HBZ peptide stimulation. HBZ specific T cell response was also observed in rVV-HBZ-immunized Rhesus macaque. These HBZ-specific CTLs were produced effector cytokines by HBZ157-176 peptide stimulation. Furthermore we generated human HBZ specific CTLs from HLA-A2 and HLA-A24 positive healthy donor using HBZ157-176 peptide. The HBZ specific CTLs produce effector cytokines when stimulated with HBZ157-176 peptide-pulsed BLCL. This study suggests that HBZ specific CTLs could be useful for ATL treatment.
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Report
(3 results)
Research Products
(7 results)
