Design of vaccine antigen focused on the epitopes recognized by broad neutralizing antibodies

Research Project

Project/Area Number	26860304
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Virology
Research Institution	一般財団法人阪大微生物病研究会
Principal Investigator	Inoue Yuji 一般財団法人阪大微生物病研究会, 研究部探索研究課, 主任 (90548869)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	ワクチン / インフルエンザ / エピトープ / ウイルス
Outline of Final Research Achievements	In this study, we attempted to construct a vaccine complex to protect infection from a broad range of influenza viral strains. Here we focused on the "stem region" in the hemagglutinin protein on the surface of influenza virion. The stem region is known to be less-variable but less-accessible compared to another part called "head region". To increase accessibility of the stem region, we prepared recombinant HA proteins and tried to bind them onto the surface of nanoparticles through the head region, on which HA molecules are inverted to expose the stem region.