The role of C-type lectin in Chikungunya arthritis

• Project/Area Number: 26860308
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Nakayama Eri 国立感染症研究所, ウイルス第一部, 研究員 (40645339)
• Research Collaborator: Suhrbier Andreas QIMR Berghofer Medical Research Institute ; Prow Natalie QIMR Berghofer Medical Research Institute
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: チクングニアウイルス / 関節炎 / レクチン / 糖鎖

Outline of Final Research Achievements

Chikungunya virus is transmitted to human by mosquitoes. The most common symptoms of Chikungunya virus infection are fever and arthritis. Here we showed that C-type lectins, which bind to carbohydrates in a calcium-dependent manner, bound to Chikungunya virus and mediated the attachment of the virus to the cell surface. Our data suggested that Chikungunya virus attached to the cells expressing different C-type lectins using the different glycan structures on the viral glycoprotein added in the cycle between mosquito and mammalian hosts. Furthermore, we developed the mouse model of Chikungunya arthritis seen in patients using the viral isolates from Chikungunya imported cases. The gene expression of the lectin-related molecules was upregulated in the mouse lower legs showing the arthritis by Chikungunya virus infection. These results implied that the Chikungunya viral attachment to the cell surface might increase by upregulated lectin-related molecules at the site of arthritis.

Research Products

• [Int'l Joint Research] QIMR Medical Research Institute(オーストラリア)
• [Int'l Joint Research] QIMR Medical Research Institute(オーストラリア)
• [Journal Article] Importation of Zika Virus from Vietnam to Japan, November 2016. (2017)
  • Author(s): Hashimoto T, Kutsuna S, Tajima S, Nakayama E, Maeki T, Taniguchi S, Lim CK, Katanami Y, Takeshita N, Hayakawa K, Kato Y, Ohmagari N.
  • Journal Title: Emerg Infect Dis. Volume: 23 Issue: 7 Pages: 1223-1225
  • DOI: 10.3201/eid2307.170519
  • Peer Reviewed / Open Access
• [Journal Article] Chikungunya Fever in Traveler from Angola to Japan, 2016. Emerg Infect Dis. (2017)
  • Author(s): Takaya S, Kutsuna S, Nakayama E, Taniguchi S, Tajima S, Katanami Y, Yamamoto K, Takeshita N, Hayakawa K, Kato Y, Kanagawa S, Ohmagari N.
  • Journal Title: Emerg Infect Dis. Volume: 23 Issue: 1 Pages: 156-158
  • DOI: 10.3201/eid2301.161395
  • Peer Reviewed / Open Access
• [Journal Article] A case of consecutive infection with Zika virus and Chikungunya virus in Bora Bora, French Polynesia. (2017)
  • Author(s): Kutsuna S, Kato Y, Nakayama E, Taniguchi S, Takasaki T, Yamamoto K, Takeshita N, Hayakawa K, Kanagawa S, Ohmagari N
  • Journal Title: J Infect Chemother. Volume: 23 Issue: 2 Pages: 114-116
  • DOI: 10.1016/j.jiac.2016.08.003
  • Peer Reviewed / Open Access
• [Journal Article] Whole Genome Sequencing-Based Molecular Epidemiologic Analysis of Autochthonous Dengue Virus Type 1 Strains Circulating in Japan in 2014 (2017)
  • Author(s): Tajima S, Nakayama E, Kotaki A, Moi ML, Ikeda M, Yagasaki K, Saito Y, Shibasaki KI, Saijo M, Takasaki T.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 1 Pages: 45-49
  • DOI: 10.7883/yoken.JJID.2016.086
  • NAID: 130005296393
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access
• [Journal Article] Autochthonous Chikungunya Fever in Traveler Returning to Japan from Cuba. (2016)
  • Author(s): Tsuboi M, Kutsuna S, Kato Y, Nakayama E, Shibasaki K, Tajima S, Takasaki T, Katanami Y, Yamamoto K, Takeshita N, Hayakawa K, Kanagawa S, Ohmagari N.
  • Journal Title: Emerg Infect Dis. Volume: 22 Issue: 9 Pages: 1683-1685
  • DOI: 10.3201/eid2209.160603
  • Peer Reviewed / Open Access
• [Journal Article] 蚊が媒介するウイルス感染のわが国への脅威と今後(デング、チクングンヤ、ジカ) (2016)
  • Author(s): 中山絵里、高崎智彦
  • Journal Title: 感染・炎症・免疫 Volume: 46
• [Journal Article] 先天性ジカウイルス感染症 (2016)
  • Author(s): 中山絵里、高崎智彦
  • Journal Title: 成人病と生活習慣病 Volume: 46 Pages: 1407-1410
• [Journal Article] クリミア・コンゴ出血熱 (2016)
  • Author(s): 中山絵里、西條政幸
  • Journal Title: 感染症 Volume: 272
• [Journal Article] Chikungunya Fever in Japan Imported from the Caribbean Islands (2016)
  • Author(s): Imai K, Nakayama E, Maeda T, Mikita K, Kobayashi Y, Mitarai A, Honma Y, Miyake S, Kaku K, Miyahira Y, Kawana A.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 69 Issue: 2 Pages: 151-153
  • DOI: 10.7883/yoken.JJID.2015.063
  • NAID: 130005138550
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access
• [Journal Article] In vitro growth, pathogenicity and serological characteristics of the Japanese encephalitis virus genotype V Muar strain. (2015)
  • Author(s): 3.Tajima S, Yagasaki K, Kotaki A, Tomikawa T, Nakayama E, Moi ML, Lim CK, Saijo M, Kurane I, Takasaki T.
  • Journal Title: J Gen Virol. Volume: 96 Issue: 9 Pages: 2661-2669
  • DOI: 10.1099/vir.0.000213
  • Peer Reviewed / Open Access
• [Journal Article] Seroepidemiological prevalence of multiple species of filoviruses in fruit bats (Eidolon helvum) migrating in Africa. (2015)
  • Author(s): Hirohito Ogawa, Hiroko Miyamoto, Eri Nakayama, Reiko Yoshida, Ichiro Nakamura, Hirofumi Sawa, Akihiro Ishii, Yuka Thomas, Emiko Nakagawa, Keita Matsuno, Masahiro Kajihara, Junki Maruyama, Manabu Igarashi, Kimihito Ito, Ayato Takada et al
  • Journal Title: Journal of Infectious Diseases Volume: jiv063 Issue: suppl 2 Pages: S101-S108
  • DOI: 10.1093/infdis/jiv063
  • Peer Reviewed / Open Access
• [Journal Article] チクングニア熱 (2015)
  • Author(s): 中山絵里、高崎智彦
  • Journal Title: 臨床と微生物 Volume: 42
• [Journal Article] デング熱 (2015)
  • Author(s): 中山絵里、高崎智彦
  • Journal Title: 化学療法の領域 Volume: 6月号
• [Journal Article] エボラウイルス感染症 (2015)
  • Author(s): 中山絵里、西條政幸
  • Journal Title: INFECTION FRONT Volume: 34
• [Journal Article] Identification and amplification of Japanese encephalitis virus and Getah virus propagated from a single porcine serum sample: a case of coinfection. (2014)
  • Author(s): Tajima S, Kotaki A, Yagasaki K, Taniwaki T, Moi ML, Nakayama E, Saijo M, Kurane I, Takasaki T.
  • Journal Title: Arch Virol. Volume: 159 Issue: 11 Pages: 2969-75
  • DOI: 10.1007/s00705-014-2152-x
  • Peer Reviewed
• [Presentation] 日本脳炎ウイルス流行株の把握と病原性・ワク チン感受性評価系の確立 (2016)
  • Author(s): 中山絵里、田島茂、谷ヶ崎和美、小滝徹、富川拓海、 モイメンリン、林昌宏、西僚政幸、倉根一郎、高崎智彦
  • Organizer: 第119回 日本小児科学会学術集会
  • Place of Presentation: 北海道
  • Year and Date: 2016-05-13
• [Presentation] IFNAR1欠損マウスにおけるジカウイルスの病原性解析 (2016)
  • Author(s): 中山絵里
  • Organizer: トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 北海道
• [Presentation] Complete nucleotide sequences of two groups of autochthonous DENV-1 strains in Japan, 2014. (2015)
  • Author(s): Tajima S, Tomikawa T, Nakayama E, Kotaki A, Moi ML, Ikeda M, Yagasaki K, Saito Y, Shibasaki K, Saijo M, Takasaki T.
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Next-Generation Sequencing of Dengue Virus Genome. (2015)
  • Author(s): 中山絵里
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-21
• [Presentation] デング熱について (2015)
  • Author(s): 中山絵里
  • Organizer: 平成26年度獣医公衆衛生講習会
  • Place of Presentation: 北海道獣医師会館、北海道
  • Year and Date: 2015-01-07
  • Invited
• [Presentation] チクングニア熱の輸入症例の報告および血清学的診断法の開発 (2014)
  • Author(s): 中山絵里、小滝徹、谷ヶ崎和美、林昌宏、西條政幸、高崎智彦
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] 遺伝子型Ⅴ型日本脳炎ウイルス株に対する日本脳炎ワクチンの中和効果 (2014)
  • Author(s): 田島茂、谷ヶ崎和美、小滝徹、中山絵里、Moi Meng Ling、林昌宏、西條政幸、倉根一郎、高崎智彦
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2014-11-10 – 2014-11-12
• [Remarks] 国立感染症研究所ウイルス第一部第二室
  • URL: http://www0.nih.go.jp/vir1/NVL/NVL.html
• [Remarks] チクングニア熱 -Chikungunya fever -
  • URL: http://www0.nih.go.jp/vir1/NVL/Aiphavirus/Chikungunyahtml.htm
• [Remarks] デングウイルス感染症情報
  • URL: http://www0.nih.go.jp/vir1/NVL/NVL.html