Sialoglycan facilitate cancer escape from immunosurveillance via sialic acid-binding Siglec-7 on NK cells
| Project/Area Number |
26860320
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Hashimoto Noboru 名古屋大学, 医学(系)研究科(研究院), 研究機関研究員 (90712365)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 糖鎖 / 癌 / 免疫 / 免疫監視機構 / 質量分析 / シアル酸 |
|---|
| Outline of Final Research Achievements |
Siglec-7 is an inhibitory receptor expressed on NK cells and monocytes and transduces inhibitory signals into immune cells by binding to its ligands. Although a few ligands have been reported, specificity of Siglec-7-recognized structures has not been clarified. To solve this issue, we established several sialyltransferase-transfectants that showed definite binding of Siglec-7-Fc using a human colon cancer cell line. By using the transfectants, we clarified o-glycan is a new ligand of Siglec-7. While NK cells showed high cytotoxic activity toward the parent cells, reduced cytotoxicity was observed for the transfectants. In addition, ITIM of Siglec-7 was strongly phosphorylated in the transfectants. As for the transfectants, Siglec-7-binding enhanced cell migration. Taken together, we suggest that interaction between Siglec-7 and O-glycan enable cancer cells to escape from immunosurveillance by suppression of immune cells and enhancement of cancer malignancy.
|
Report
(3 results)
Research Products
(14 results)
-
[Journal Article] Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity2015
Author(s)
Kohki Matsubara, Yoshihiro Matsushita, Kiyoshi Sakai, Fumiya Kano, Megumi Kondo, Mariko Noda, Noboru Hashimoto, Shiro Imagama, Naoki Ishiguro, Akio Suzumura, Minoru Ueda, Koichi Furukawa, Akihito Yamamoto
-
Journal Title
Journal of Neuroscience
Volume: 35
Issue: 6
Pages: 2452-2464
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
