Development of in vitro culture to generate disease-related-antigen-reactive cytotoxic T lymphocytes using iPSC technology
| Project/Area Number |
26860324
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
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| Research Institution | Kyoto University |
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Principal Investigator |
Kawai Yohei 京都大学, iPS細胞研究所, 研究員 (90623364)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | iPS細胞 / CTL / iPSC / adaptive lymphoid cell / naive CTL / CD8alphabeta / Cytotoxic T cell / フィーダーフリー拡大培養 |
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| Outline of Final Research Achievements |
Adoptive transfer of cytotoxic T lymphocytes (CTLs) is highly promising against cancer and chronic infection, however in conventional protocol of CTL preparation, cell exhaustion is inevitable and critically hampers production of sufficient number of CTLs. Thus we developed novel iPSC-technology-based system to regenerate CTLs in cost- and labor-effective manner and successfully produced CTLs, whose cytotoxic activity was equivalent to that of original cells.
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Report
(3 results)
Research Products
(4 results)
