Development of in vitro culture to generate disease-related-antigen-reactive cytotoxic T lymphocytes using iPSC technology

Research Project

Project/Area Number	26860324
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Immunology
Research Institution	Kyoto University
Principal Investigator	Kawai Yohei 京都大学, iPS細胞研究所, 研究員 (90623364)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	iPS細胞 / CTL / iPSC / adaptive lymphoid cell / naive CTL / CD8alphabeta / Cytotoxic T cell / フィーダーフリー拡大培養
Outline of Final Research Achievements	Adoptive transfer of cytotoxic T lymphocytes (CTLs) is highly promising against cancer and chronic infection, however in conventional protocol of CTL preparation, cell exhaustion is inevitable and critically hampers production of sufficient number of CTLs. Thus we developed novel iPSC-technology-based system to regenerate CTLs in cost- and labor-effective manner and successfully produced CTLs, whose cytotoxic activity was equivalent to that of original cells.

Report

(3 results)