| Project/Area Number |
26860333
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腸管免疫 / 囲食膜 / トランスグルタミナーゼ / ショウジョウバエ / 外毒素プロテアーゼ / 経口感染 / タンパク質架橋 / キイロショウジョウバエ / TGase / IMD経路 / 自然免疫 / 免疫寛容 / 架橋反応 / 活性酸素 |
|---|
| Outline of Final Research Achievements |
Intestinal homeostasis is ensured by a subtle balance between bacteria and host immunity. Gut epithelial barriers, such as the mucus layer in mammals and the peritrophic matrix in invertebrates, have a protective function for hosts, as they are impermeable to the invading intestinal microbes. In this study, we discovered, in the fly Drosophila melanogaster, that transglutaminase, known to be molecular glue involved in protein-protein covalent bond formation, is essential for peritrophic matrix formation to convert a peritrophic protein Drosocrystallin into a stable fiber-like structure and to inhibit the pathogenesis against pathogenic bacteria. Knockdown of transglutaminase gene, the permeability of the peritrophic matrix was increased and the fly exhibited highly susceptibility against a bacterial toxic protease. Transglutaminase contributes to form the stable mesh-like barrier on the peritrophic matrix and tolerant for pathogenic microorganisms.
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