The development of the anti-angiogenic factor VEGF-A165b quantification methods for cardiovascular disease.
Project/Area Number |
26860367
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
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Research Institution | Nagoya University |
Principal Investigator |
KIKUCHI RYOSUKE 名古屋大学, 医学部附属病院, 臨床検査技師 (30721435)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | VEGF-A / 臨床検査医学 / VEGF-A165b / 血管新生 / 抗血管新生因子 / 微小血管障害 / マウス下肢虚血モデル / 代謝障害 |
Outline of Final Research Achievements |
The VEGF-A165 (pro-angiogenic) and VEGF-A165b (anti-angiogenic), only the sequence of 6 amino acid residues of the C- terminal side is different. The current ELISA system of VEGF-A does not discriminate between different VEGF-A isoforms (pro- or anti-angiogenic forms). Here, we made the development of VEGF-A165b specific ELISA system, and the clarified the molecular mechanisms of VEGF-A165b. Moreover, the urinary VEGF-A165b concentration was found to be useful for the development assessment of renal dysfunction.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] An antiangiogenic isoform of VEGF-A contributes to impaired vascularization in peripheral artery disease.2014
Author(s)
Kikuchi R, Nakamura K, MacLauchlan S, Ngo DT, Shimizu I, Fuster JJ, Katanasaka Y, Yoshida S, Qiu Y, Yamaguchi TP, Matsushita T, Murohara T, Gokce N, Bates DO, Hamburg NM, Walsh K
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Journal Title
Nat Med
Volume: 20(12)
Issue: 12
Pages: 1464-71
DOI
Related Report
Peer Reviewed
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[Journal Article] Angiotensin II receptor blocker ameliorates stress-induced adipose tissue inflammation and insulin resistance.2014
Author(s)
Hayashi M, Takeshita K, Uchida Y, Yamamoto K, Kikuchi R, Nakayama T, Nomura E, Cheng XW, Matsushita T, Nakamura S, Murohara T.
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Journal Title
PLoS One
Volume: 9
Issue: 12
Pages: e116163-e116163
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Anti-angiogenic actions of VEGF-A165b, a novel isoform of VEGF-A, in human obesity2014
Author(s)
Doan TM, Farb M, Kikuchi R, Karki S, Tiwari S, Bigornia S, Bates DO, Hamburg NM, Vita JA, Hess DT, Walsh K, Gokce N
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Journal Title
Circulation
Volume: 130
Issue: 13
Pages: 1072-1080
DOI
Related Report
Peer Reviewed
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[Journal Article] Association of circulating C1q/TNF-related protein 1 levels with coronary artery disease in men.2014
Author(s)
Yuasa D, Ohashi K, Shibata R, Takeshita K, Kikuchi R, Takahashi R, Kataoka Y, Miyabe M, Joki Y, Kambara T, Uemura Y, Matsuo K, Hayakawa S, Hiramatsu-Ito M, Ito M, Ikeda N, Murohara T, Ouchi N.
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Journal Title
PLoS One
Volume: 8
Issue: 6
Pages: e99846-e99846
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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