The development of new examination in autoimmune thyroid disease used by Toll- like receptors

• Project/Area Number: 26860369
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Osaka University
• Principal Investigator: Inoue Naoya 大阪大学, 医学部附属病院, 臨床検査技師 (80710269)
• Research Collaborator: IWATANI Yoshinori 大阪大学, 大学院 医学系研究科 保健学専攻 生体情報科学講座, 教授 ; WATANABE Mikio 大阪大学, 大学院 医学系研究科 保健学専攻 生体情報科学講座, 准教授 ; HIDAKA Yoh 大阪大学, 医学部附属病院臨床検査部
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: TLR / 遺伝子多型 / 発現解析 / 検査法 / TLR7 / TLR9 / 自己免疫性甲状腺疾患 / 臨床検査 / polymorphism / 臨床検査法の開発

Outline of Final Research Achievements

In the TLR4 rs41426344 polymorphism, GC + CC genotypes and C allele were more frequent in the mild HD group compared to the severe HD group.The ratio of intracellular TLR7 and intracellular TLR9 intensities in B cells was lower in patients with GD in remission than in patients with intractable GD.In conclusion, the GC + CC genotypes of the TLR4 rs41426344 polymorphism protect against thyroid destruction and are less prone to hypothyroidism.The ratio of intracellular TLR7 and intracellular TLR9 intensities in B cells was associated with the development and intractability of GD and UNC93B1 polymorphisms were associated with the development of GD.

Research Products

• [Journal Article] Polymorphisms in and expression of Toll-like receptors in autoimmune thyroid diseases. (2016)
  • Author(s): Inoue, N., Katsumata, Y., Watanabe, M., Ishido, N., Manabe, Y., Watanabe, A., Masutani, R., Hidaka, Y., Iwatani, Y.
  • Journal Title: Autoimmunity Volume: 26 Issue: 3 Pages: 1-10
  • DOI: 10.1080/08916934.2016.1261835
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association between functional polymorphisms in the Toll-like receptor 4 (TLR4) gene and HD severity. (2015)
  • Author(s): Inoue, N., Watanabe, M., Katsumata, Y., Ishido, N., Hidaka, Y., Iwatani, Y
  • Journal Title: Tissue Antigens Volume: 85 Issue: 3 Pages: 209-211
  • DOI: 10.1111/tan.12518
  • Peer Reviewed
• [Presentation] The associations between TLR7 polymorphisms and autoimmune thyroid disease (2016)
  • Author(s): Inoue, N., Watanabe, M., Katsumata, Y, Ishido, N., Hidaka, Y., Iwatani, Y.
  • Organizer: AACC Annual Meeting & Clinical Lab Exp
  • Place of Presentation: Philadelphia, America
  • Int'l Joint Research
• [Presentation] Association between functional polymorphisms in the Toll-like receptor 4 (TLR4) gene and HD severity (2015)
  • Author(s): Naoya Inoue, Mikio Watanabe, Yuka Katsumata, Naoko Ishido, Yoh Hidaka, Yoshinori Iwatani
  • Organizer: AACC Annual Meeting & Clinical Lab Expo 2015
  • Place of Presentation: Atlanta, America
  • Year and Date: 2015-07-28
  • Int'l Joint Research