| Project/Area Number |
26860373
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Research Collaborator |
UMEZU Tomohiro 東京医科大学, 医学部, 講師 (40385547)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 移植片対宿主病 / 造血幹細胞移植 / エクソソーム / miRNA / リキッドバイオプシー |
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| Outline of Final Research Achievements |
Exosomes, small endosome-derived extracellular vesicles, are present in various biological fluids, such as plasma, thus have potential in facilitating molecular diagnosis. The aim of this study is to determine clinically relevant exosomal miRNAs in patients developing LA GVHD after HSCT.
We evaluated blood samples obtained from 10 patients who underwent HSCT for treatment of hematologic diseases. Exosomal miRNA profiling was done using a TaqMan low-density array.
We found that a subset of miRNAs was significantly up-regulated in the exosomes of LA GVHD groups compared to non-GVHD controls. Accordingly, the predicted target genes of altered miRNAs were extracted by MirTarBase. To estimate the biological implication of LA GVHD related miRNAs, we then investigated comprehensively possible related pathway using DAVID bioinformatics tools. Our study indicates that altered expression of certain exosomal miRNAs may play an important role in the immunoregulation of LA GVHD.
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