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The effects of NKT cell-mediated immunotherapy by IDO inhibitor in mouse lung metastasis model

Research Project

Project/Area Number 26860376
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionFujita Health University (2016)
Suzuka University of Medical Science (2014-2015)

Principal Investigator

Hoshi Masato  藤田保健衛生大学, 医療科学部, 講師 (40633996)

Research Collaborator SEISHIMA Mitsuru  
SAITO Kuniaki  
ITO Hiroyasu  
HARA Akira  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsインドールアミン酸素添加酵素1 / インドールアミン酸素添加酵素2 / トリプトファン代謝産物 / B16F10細胞 / 抗腫瘍効果 / 細胞免疫療法 / IDO1 / IDO2 / B16F10 / IDO1 / IDO2 / B16F10 / α-ガラクトシルセラミド / トリプトファン代謝 / 1-MT / IDO / B16細胞 / α-ガラクトシルセラミド / トリプタファン代謝 / 1-metyl-D-tryptophan / 1-metyl-L-tryptophan
Outline of Final Research Achievements

The role of indoleamine 2,3-dioxygenase (Ido) in the L-tryptophan (Trp)-kynurenine (Kyn) pathway after lung metastasis model by injecting B16F10 cells was investigated. We used α-GalCer administrated mice of wild type (WT), IDO1 KO, IDO2 KO, and mice treated with 1-methyl-D or L-tryptophan (D or L-1MT), an inhibitor of Ido1 or Ido2 respectively, to study the importance of Trp-Kyn pathway metabolites.
The levels of Ido1 and Ido2 mRNA and protein expression in the lung from WT mice were significantly higher than those from non-injected WT mice. The anti-tumor effect in the lung from IDO1 KO mice or L-1MT treated mice was markedly improved compared to that in WT mice, IDO2 KO mice, and D-1MT treated mice. Moreover, the levels of Trp metabolites in lung from IDO1 KO mice or L-1MT treated mice was significantly decreased compared to that in lung from WT mice, IDO2 KO mice, and D-1MT treated mice.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Absence of kynurenine 3-monooxygenase reduces mortality of acute viral myocarditis in mice2017

    • Author(s)
      Kubo H, Hoshi M, Mouri A, Tashita C, Yamamoto Y, Nabeshima T, Saito K.
    • Journal Title

      IMMUNOLOGY LETTERS

      Volume: 181 Pages: 94-100

    • DOI

      10.1016/j.imlet.2016.11.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhibition of indoleamine 2,3-dioxygenase 1 expression alters immune response in colon tumor microenvironment in mice.2015

    • Author(s)
      Takamatsu M, Hirata A, Ohtaki H, Hoshi M, Ando T, Ito H, Hatano Y, Tomita H, Kuno T, Saito K, Seishima M, Hara A.
    • Journal Title

      Cancer Sci.

      Volume: 106(8) Issue: 8 Pages: 1008-15

    • DOI

      10.1111/cas.12705

    • NAID

      120005767026

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Early microlesion of viral encephalitis confirmed by galectin-3 expression after a virus inoculation.2015

    • Author(s)
      Kobayashi K, Niwa M, Hoshi M, Saito K, Hisamatsu K, Hatano Y, Tomita H, Miyazaki T, Hara A.
    • Journal Title

      Neuroscience letters.

      Volume: 592 Pages: 107-112

    • DOI

      10.1016/j.neulet.2015.02.061

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blockade of indoleamine 2,3-dioxygenase reduces mortality from peritonitis and sepsis in mice by regulating functions of CD11b+ peritoneal cells.2014

    • Author(s)
      Hoshi M, Osawa Y, Ito H, Ohtaki H, Ando T, Takamatsu M, Hara A, Saito K, Seishima M.
    • Journal Title

      Infect Immun.

      Volume: 82(11) Issue: 11 Pages: 4487-95

    • DOI

      10.1128/iai.02113-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Kynurenine production mediated by indoleamine 2, 3-dioxygenase aggravates liver injury in HBV-specific CTL-induced fulminant hepatitis2014

    • Author(s)
      Ohtaki H, Ito H, Ando K, Ishikawa T, Hoshi M, Ando T, Takamatsu M, Hara A, Moriwaki H, Saito K, Seishima M
    • Journal Title

      Biochim Biophys Acta

      Volume: 1842(9) Issue: 9 Pages: 1464-71

    • DOI

      10.1016/j.bbadis.2014.04.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Indoleamine 2,3-dioxygenase 1 is upregulated in activated microglia in mice cerebellum during acute viral encephalitis2014

    • Author(s)
      Taguchi A, Niwa M, Hoshi M, Saito K, Masutani T, Hisamatsu K, Kobayashi K, Hatano Y, Tomita H, Hara A
    • Journal Title

      Neurosci Lett

      Volume: 3564 Pages: 120-5

    • DOI

      10.1016/j.neulet.2014.01.051

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス致死性敗血症モデルにおけるインドールアミン酸素添加酵素の役割について2016

    • Author(s)
      星 雅人 久保 緋紗子 毛利 彰宏 勅使河原 知明 山菅 和可奈 和田 直也 山本 康子 鍋島 俊隆 齋藤 邦明
    • Organizer
      第37回日本トリプトファン研究会学術集会
    • Place of Presentation
      東京農業大学、東京都世田谷区
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Absence of kynurenine 3-monooxygenase reduces mortality of acute viral myocarditis in mice2016

    • Author(s)
      久保 緋紗子 星 雅人 毛利 彰宏 勅使河原 知明 山菅 和可奈 和田 直也 山本 康子 鍋島 俊隆 齋藤 邦明
    • Organizer
      第37回日本トリプトファン研究会学術集会
    • Place of Presentation
      東京農業大学、東京都世田谷区
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] A rapid and highly sensitive method to evaluate the quinolinic acid using recombinant enzymes with HPLC-Fluorescence detection2015

    • Author(s)
      Hidetsugu Fujigaki, Akiko Sakamoto-Morise, Suwako Fujigaki, Kanako Hirabayashi-Takahashi, Masato Hoshi, Yasuko Yamamoto, Sanford P. Markey, and Kuniaki Saito
    • Organizer
      14th International Society for Tryptophan Research
    • Place of Presentation
      Grand Rapids, USA
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Blockade of Indoleamine 2,3-dioxygenase reduces mortality from peritonitis and sepsis in mice by regulating function of CD11b+ peritoneal cells2015

    • Author(s)
      Masato Hoshi, Yosuke Osawa, Hiroyasu Ito, Mituru Seishima, Kuniaki Saito
    • Organizer
      14th International Society for Tryptophan Research
    • Place of Presentation
      Grand Rapids, USA
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 論文1

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556390/

    • Related Report
      2015 Research-status Report
  • [Remarks] 論文2

    • URL

      http://www.sciencedirect.com/science/article/pii/S0304394015001767

    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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