Development of an enzymatic assay for the determination of kynurenine
| Project/Area Number |
26860379
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | トリプトファン代謝 / キヌレニン / うつ病 |
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| Outline of Final Research Achievements |
Kynurenine (KYN) is a metabolite of L-tryptophan. An increased KYN concentration in the blood and cerebrospinal fluid is associated with the pathogenesis of depression. The purpose of this study is to develop an enzymatic assay for the determination of KYN as a diagnostic marker for depression. We have succeeded in purifying KYN-metabolizing enzymes; kynurenine 3-monooxygenase and kynurenine aminotransferase2, using baculovirus expression system. Furthermore, we have succeeded in developing an enzymatic assay for KYN using the recombinant enzymes.
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Report
(3 results)
Research Products
(5 results)
