| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Kynurenine (KYN) is a metabolite of L-tryptophan. An increased KYN concentration in the blood and cerebrospinal fluid is associated with the pathogenesis of depression. The purpose of this study is to develop an enzymatic assay for the determination of KYN as a diagnostic marker for depression. We have succeeded in purifying KYN-metabolizing enzymes; kynurenine 3-monooxygenase and kynurenine aminotransferase2, using baculovirus expression system. Furthermore, we have succeeded in developing an enzymatic assay for KYN using the recombinant enzymes.
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