Comprehensive analysis of acute encephalitis with unknown aetiology in children.
| Project/Area Number |
26860418
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Epidemiology and preventive medicine
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| Research Institution | Oita University |
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Principal Investigator |
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| Research Collaborator |
AHMED Kamruddin マレーシア国立サバ大学, 医学部, 教授
MORI Daisuke マレーシア国立サバ大学, 医学部, 講師
FUJIMOTO Tamotsu 大分こども病院, 院長
NISHIZONO Akira 大分大学, 医学部・微生物学講座, 教授
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 小児急性脳炎 / ウイルス感染症 / 原因不明脳炎 / 遺伝子診断 / 分子疫学 / 大分県 / 次世代シーケンス / ウイルス性脳炎 / 遺伝子検査 |
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| Outline of Final Research Achievements |
More than half of cases of encephalitis remain unknown. We examined the causative virus in cerebrospinal fluid samples from encephalitis children with unknown aetiology by RT-PCR. The nucleotide sequence of viral gene was identified by direct-sequencing of PCR products and phylogenetic analysis was conducted. Human adenovirus, Human herpesvirus, Human enterovirus, Human parechovirus, Human bocavirus and Human echovirus were detected in cerebrospinal fluid samples from encephalitis children. It indicated these viruses might be considered as the causative pathogen of encephalitis in children in Oita, Japan. Moreover, we evaluated high-sensitivity detection method for viral encephalitis using next generation sequencing.
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Report
(5 results)
Research Products
(5 results)
