Investigation of the effect of arsenic on the cholesterol metabolism

• Project/Area Number: 26860445
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Kagoshima Women's Junior College (2015-2016); Jikei University School of Medicine (2014)
• Principal Investigator: Takumi Shota 鹿児島女子短期大学, その他部局等, 准教授 (80570770)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヒ素 / ABCA1 / コレステロール / トランスポーター

Outline of Final Research Achievements

Recent epidemiological studies suggest that arsenic exposure involved in atherosclerosis. In this study, we focused on the effect of arsenic on the cholesterol metabolism by using Hepa1c1c7 and HepG2 cells. The gene expression and Western Blot analysis showed that arsenic suppress the expression of ATP-binding cassette transporter-A1 (ABCA1) which involved in HDL biogenesis. Furthermore, arsenic induced the accumulation of cellular cholesterol. These results suggest that arsenic disrupt the cholesterol efflux through the inhibition of the expression of ABCA1.

Research Products

• [Journal Article] Overexpression of Carboxylesterase Contributes to the Attenuation of Cyanotoxin Microcystin-LR Toxicity (2017)
  • Author(s): Shota Takumi, Tai Shimono, Satoshi Ikema, Yuki Hotta, Petros Kingstone Chigwechokha, Kazuhiro Shiozaki, Yasumasa Sugiyama, Mitsuru Hashimoto, Tatsuhiko Furukawa, Masaharu Komatsu
  • Journal Title: Comparative Biochemistry and Phyisiology part-C Volume: 194 Pages: 22-27
  • DOI: 10.1016/j.cbpc.2017.01.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Augmenting effects of gestational arsenite exposure of C3H mice on the hepatic tumors of the F2 male offspring via the F1 male offspring (2015)
  • Author(s): Nohara K, Okamura K, Suzuki T, Murai H, Ito T, Shinjo K, Takumi S, Michikawa T, Kondo Y, Hata K.
  • Journal Title: J Appl Toxicol Volume: 印刷中 Issue: 1 Pages: 105-112
  • DOI: 10.1002/jat.3149
  • Peer Reviewed / Open Access
• [Journal Article] Okadaic acid is taken-up into the cells mediated by human hepatocytes transporter OATP1B3. (2015)
  • Author(s): Ikema S, Takumi S, Maeda Y, Kurimoto T, Bohda S, Chigwechokha PK, Sugiyama Y, Shiozaki K, Furukawa T, Komatsu M.
  • Journal Title: Food and Chemical Toxicology Volume: 83 Pages: 229-236
  • DOI: 10.1016/j.fct.2015.06.006
  • Peer Reviewed
• [Journal Article] Naringin attenuates the cytotoxicty of hepatotoxin microcystin-LR by the curious mechanisms to OATP1B1- and OATP1B3-expressing cells. (2015)
  • Author(s): Takumi, S., Ikema, S., Hanyu, T., Shima, Y., Kurimoto, T., Shiozaki, K., Sugiyama, Y., Park, H., Ando, S., Furukawa, T., Komatsu, M.
  • Journal Title: Environ Toxicol Pharmacol Volume: 39 Issue: 2 Pages: 974-981
  • DOI: 10.1016/j.etap.2015.02.021
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Microcystin-LR Induces Anoikis Resistance to the Hepatocyte Uptake Transporter OATP1B3-expressing Cell Lines. (2014)
  • Author(s): Takano, H., Takumi, S., Ikema, S., Mizoue, N., Hotta, Y., Shiozaki, K., Sugiyama, Y., Furukawa, T., Komatsu, M.
  • Journal Title: Toxicology Volume: 326 Pages: 53-61
  • DOI: 10.1016/j.tox.2014.10.003
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ヒ素曝露による細胞内コレステロールの蓄積 (2017)
  • Author(s): 内匠正太、山下優香、小松正治、柳澤裕之
  • Organizer: 第87回日本衛生学会学術総会
  • Place of Presentation: 宮崎
  • Year and Date: 2017-03-26
• [Presentation] ヒ素がコレステロール代謝に及ぼす影響 (2016)
  • Author(s): 内匠正太、小松正治、柳澤裕之
  • Organizer: 第86回日本衛生学会学術総会
  • Place of Presentation: 旭川市民文化会館
  • Year and Date: 2016-05-11
• [Presentation] 亜鉛欠乏及び過剰食が長寿遺伝子Sirt1の発現に及ぼす影響 (2015)
  • Author(s): 内匠正太、木戸尊將、関良子、与五沢真吾、須賀万智、柳澤裕之
  • Organizer: 第88回日本産業衛生学会
  • Place of Presentation: グランフロント大阪
  • Year and Date: 2015-05-13
• [Presentation] Gestational Arsenic Exposure Affects Gene Expression in the Kidney and Lung in the F1 and F2 Mice (2015)
  • Author(s): Takumi S, Okamura K, Suzuki T, Hano H, Nohara K
  • Organizer: The 54th Annual Meeting of the Society of Toxicology
  • Place of Presentation: 米国, サンディエゴ, コンベンションセンター
  • Year and Date: 2015-03-22 – 2015-03-26