| Project/Area Number |
26860450
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene and public health
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ポリオウイルス / 環境サーベイランス / レセプター結合型ビーズ / 次世代シークエンス / ポリオ / 直接検出 / サーベイランス |
|---|
| Outline of Final Research Achievements |
BY using negatively-charged filter and magnetic nanobeads with PV receptor (PVR-MB), we could concentrate spiked PV to 5,000-fold conc. Besides this, entire capsid coding region amprification (ECRA) method and NGS analysis were highest sensitive at PV detection than any other methods. From the analysis of PV-positive Pakistani environmental samples, we could identify many kinds of PVs, including vaccine and wild strains, and other non-PV enteroviruses. It was stongly suggested that this combination method was high sensitive and time-saving PV detection tool with informative virus sequences. And this method is effective environmental PV surveillance technique without bias often found in cell culture based PV isolation.
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