One of the functional analysis for RNA-binding motif protein 3 in Vitro

• Project/Area Number: 26860464
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Shiga University of Medical Science
• Principal Investigator: MORITA SATOMU 滋賀医科大学, 医学部, 非常勤講師 (80721894)
• Co-Investigator(Renkei-kenkyūsha): MORITA Rihito 岡山理科大学, 理学部, 助教 (10613268)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: RBM3の機能解析 / RBM3の翻訳抑制 / RBM3 / 低酸素

Outline of Final Research Achievements

We previously reported that RBM3 in the myocardium using immunohistochemical staining, and the appearance patterns changed by environment of agonal phase. We investigated the function of RBM3 in Vitro.
Our results was different from previous in Vivo investigations. Our results from the in Vitro cell-free translation system showed that RBM3 inhibits translation. These findings suggest that the increase in global translation is due to the effect on miRNA processing and that inhibition of translation has little effect in Vivo.

Research Products

• [Journal Article] Histochemical Characteristics of Myocardium Obtained from Two huge Cardiomegaly with over 1000g in Weight. (2016)
  • Author(s): Furukawa S, Kataoka M, Morita S, Uno A, Hitosugi M, Matsumoto H, Nishi K.
  • Journal Title: SM J Clin Med. Volume: 2（１） Pages: 1011-1015
  • Peer Reviewed / Open Access
• [Journal Article] Expression of RNA binding protein 3 (RBM3) in the Substania Nigra might be utilized to distinguish neck compression deaths from other deaths. (2016)
  • Author(s): Nishi K, Furukawa S, Morita S, Hirosugi M, Eberle L.
  • Journal Title: Review of Albanian Legal Medicine. Volume: 12 Pages: 59-66
  • Peer Reviewed
• [Journal Article] 写真画像処理ソフトウエアを用いた小脳顆粒細胞層核密度の検討 (2016)
  • Author(s): 奥長 隼, 森田沙斗武, 西 克治, 宇野亜加里, 中川季子, 古川智之, 一杉正仁.
  • Journal Title: 法医病理 Volume: 22（2） Pages: 83-86
  • Peer Reviewed
• [Journal Article] Classification of contraction bands using immunohistochemistry (2015)
  • Author(s): Satomu Morita, Satoshi Furukawa, Katsuji Nishi
  • Journal Title: Am J Forensic Med Pathol Volume: 36 Issue: 1 Pages: 23-26
  • DOI: 10.1097/paf.0000000000000124
  • Peer Reviewed / Open Access
• [Journal Article] 心筋組織における収縮帯壊死の評価 (2015)
  • Author(s): 森田沙斗武、坂口生夫、西克治、古川智之、一杉正仁
  • Journal Title: 日本バイオレオロジー学会学会誌(B&R) Volume: 29 Pages: 102-106
  • NAID: 130006936209
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical evaluation of hypoxia markers in the myocardium (2014)
  • Author(s): Satomu Morita, Satoshi Furukawa, Kasuji Nishi
  • Journal Title: Aust J Forensic Sci Volume: 47 Issue: 2 Pages: 138-146
  • DOI: 10.1080/00450618.2014.906653
  • Peer Reviewed / Open Access
• [Presentation] 心臓マッサージによって生じるコントラクションバンドの検討 (2015)
  • Author(s): 森田沙斗武, 古川智之, 西克治, 一杉正仁
  • Organizer: 第99次日本法医学会学術全国集会
  • Place of Presentation: 高知
  • Year and Date: 2015-06-12
• [Presentation] Classification of contraction bands using immunohistochemistry (2014)
  • Author(s): 森田沙斗武
  • Organizer: 20th World Meeting of the International Association of Forensic Science
  • Place of Presentation: ソウル(大韓民国）
  • Year and Date: 2014-10-13 – 2014-10-18
• [Presentation] 収縮帯壊死とアーチファクト収縮帯の区別に対する検討 (2014)
  • Author(s): 森田 沙斗武、古川 智之、西 克治、一杉 正仁
  • Organizer: 第64回日本法医学会九州地方集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-10-10
• [Presentation] 免疫組織化学を用いたコントラクションバンドの分類 (2014)
  • Author(s): 森田沙斗武、古川智之、西克治
  • Organizer: 第98次日本法医学会学術全国集会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-16 – 2014-06-20