| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we developed MethyLight methods (quantitative real-time methylation-specific PCR) for quantitating the semen-specifically unmethylated region, DACT1 and the blood-specifically methylated region, cg06379435. The threshold methylation ratios for semen or blood identification were set by analyzing DNA from various body fluid samples using these methods. The use of these thresholds accurately identified semen or blood from other samples including highly aged semen and blood stains. Because a realtime PCR-based method requires no substantial work beyond preparing the PCR reaction mixture, simple and quick analysis may be realized with a reduced risk of contamination. Therefore, we suggest that these methods are more suited to forensic work.
|
