| Project/Area Number |
26860484
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Osaka City University |
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Principal Investigator |
TANAKA Fumio 大阪市立大学, 大学院医学研究科, 講師 (20623292)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 心身医学 / ストレス / 神経科学 / 機能性消化管障害 |
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the role of glial cell line-derived neurotrophic factor (GDNF) in duodenal epithelial barrier function. Mice were subjected to water avoidance stress as a chronic psychological stress and we investigated the duodenal protein expressions of GDNF and mRNA expressions of some kinds of cell-to-cell adhesion molecules. As a result, chronic stress suppressed the expression levels of GDNF in a time-dependent manner during the stress period. mRNA expression levels of E-cadherin, which was a kind of cell-to-cell adhesion molecules at the level of adherent junction, were significantly suppressed by the stress as well as GDNF. Other molecules did not have significant change. Furthermore, the expression levels of E-cadherin were positively correlated with the levels of GDNF. In conclusion, chronic stress down-regulated GDNF and E-cadherin, which were associated with duodenal epithelial barrier dysfunction at the level of adherent junction.
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