The alteration of microbiota and the efficiency of treatment for IBS model mice by the novel probiotics, Lactobacillus brevis SB88

• Project/Area Number: 26860490
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Asahikawa Medical College
• Principal Investigator: Ueno Nobuhiro 旭川医科大学, 医学部, 助教 (30436000)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロバイオティクス / 過敏性腸症候群 / 腸内細菌叢 / ポリリン酸 / 腸内細菌

Outline of Final Research Achievements

In this study, we investigated the alteration of microbiota of mice and the efficacy for the treatment of IBS model mice by the oral administration of heat-killed of L.brevis SB88. In result, heat-killed of L. brevis SB88 altered the microbiota of SPF mice after 28days oral administration. And it suppressed the expression of inflammatory cytokine of the epithelium cells in mice intestine, especially the mice which has been altered microbiota. Although it does not have strong effect for the IBS model mice.

Research Products

• [Journal Article] Polyphosphate, an active molecule derived from probiotic Lactobacillus brevis, improves the fibrosis in murine colitis. (2015)
  • Author(s): Kashima S, Fujiya M, Konishi H, Ueno N, Inaba Y, Moriichi K, Tanabe H, Ikuta K, Ohtake T, Kohgo Y.
  • Journal Title: Transl Res. Volume: in press Issue: 2 Pages: 00069-9
  • DOI: 10.1016/j.trsl.2015.02.002
  • NAID: 120005837997
  • Peer Reviewed / Open Access
• [Journal Article] Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway. (2015)
  • Author(s): Tanaka K, Fujiya M, Konishi H, Ueno N, Sasajima J, Moriichi K, Ikuta K, Tanabe H, Kohgo Y.
  • Journal Title: Biochem Biophys Res Commun Volume: 467 Issue: 3 Pages: 541-548
  • DOI: 10.1016/j.bbrc.2015.09.159
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Oral dietary administration of heat-killed Lactobacillus brevis SBC8803 alters the gut microbiome and ameliorates experimental colitis in mice (2014)
  • Author(s): Nobuhiro Ueno
  • Organizer: Crohn's and Colitis Foundation of America (CCFA)
  • Place of Presentation: orlando. FL. USA
  • Year and Date: 2014-12-04 – 2014-12-06