Serum Metabolomics as a Screening Method for Pancreatic Cancer

• Project/Area Number: 26860504
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kobe University
• Principal Investigator: KOBAYASHI Takashi 神戸大学, 医学(系)研究科(研究院), 研究員 (90709669)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: メタボロミクス / バイオマーカー / 血液 / 膵がん / スクリーニング / GC/MS / LC/MS / 質量分析

Outline of Final Research Achievements

The aim of this study was to discover and validate the diagnostic method using blood metabolomics. We discovered the multi-platform metabolomics system by combining the GC/MS and the LC/MS. We could measure over 600 metabolites at once by this system. Serum metabolomics using our multi-platform system showed significant change in 75 metabolites of serum in training set analysis including 14 pancreatic cancer patients and 14 controls. The diagnostic model was constructed by multivariate analysis in training set. Its sensitivity and specificity were 100% and 80%, respectively, in training set. In validation set including 44 patients and 44 controls, the sensitivity and specificity were 72.7% and 84.1%, respectively. We concluded that the serum metabolomics model might have high possibility to be a useful diagnostic method for pancreatic cancer.

Research Products

• [Journal Article] Pancreatic cancer screening using a multiplatform human serum metabolomics system (2016)
  • Author(s): Sakai A., Suzuki M., Kobayashi T., Nishiumi S., Yamanaka K., Hirata Y., Nakagawa T., Azuma T., Yoshida M
  • Journal Title: Biomarkers in Medicine Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Metabolomics for Biomarker Discovery in Gastroenterological Cancer (2014)
  • Author(s): Nishiumi S., Suzuki M., Kobayashi T., Matsubara A., Azuma T., Yoshida M.
  • Journal Title: Metabolites Volume: 4 Issue: 3 Pages: 547-571
  • DOI: 10.3390/metabo4030547
  • NAID: 120005619642
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Metabolomics evaluation of serum markers for cachexia and their intra-day variation in patients with advanced pancreatic cancer (2014)
  • Author(s): Fujiwara Y, Kobayashi T, Chayahara N, Imamura Y, Toyoda M, Kiyota N, Mukohara T, Nishiumi S, Azuma T, Yoshida M, Minami H
  • Journal Title: PLoS One Volume: 9 Issue: 11 Pages: e113259-e113259
  • DOI: 10.1371/journal.pone.0113259
  • Peer Reviewed / Open Access
• [Presentation] 血清メタボロミクスによる早期大腸がんスクリーニングシステムの開発 (2015)
  • Author(s): 小林 隆、吉田 優、東 健
  • Organizer: 第57回日本消化器病学会大会
  • Place of Presentation: グランドプリンスホテル新高輪
  • Year and Date: 2015-10-09
• [Presentation] 微量の血液からのメタボロミクスによる疾患診断 (2015)
  • Author(s): 小林 隆, 西海 信, 吉田 優
  • Organizer: メディカルジャパン2015大阪
  • Place of Presentation: インテックス大阪（大阪府・大阪市）
  • Year and Date: 2015-02-05
• [Presentation] 血清メタボローム解析による膵癌スクリーニング法の有用性 (2014)
  • Author(s): 小林 隆, 吉田 優, 東 健
  • Organizer: 第56回日本消化器病学会大会
  • Place of Presentation: 神戸国際展示場（兵庫県・神戸市）
  • Year and Date: 2014-10-25
• [Presentation] 血清メタボロミクスによる新規バイオマーカーの開発 (2014)
  • Author(s): 小林 隆
  • Organizer: 第52回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-08-28
  • Invited
• [Presentation] 血清メタボロミクスによる膵癌スクリーニング法の検討 (2014)
  • Author(s): 小林 隆, 塩見 英之, 久津見 弘, 山中 広大, 平田 祐一, 八木 洋輔, 那賀川 峻, 野村 雄大, 江崎 健, 酒井 新, 竹中 完, 増田 充弘, 有坂 好史
  • Organizer: 第45回日本膵臓学会大会
  • Place of Presentation: 北九州国際会議場(福岡県・北九州市）
  • Year and Date: 2014-07-11
• [Book] 臨床検査 メタボロミクスによる癌診断の新たな展開 (2015)
  • Author(s): 酒井 新、西海 信、小林 隆、東 健、吉田 優
  • Total Pages: 6
  • Publisher: 医学書院