Development of novel therapeutic strategies for targeting liver cancer stem cells based on the multi-omics data.
| Project/Area Number |
26860505
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 癌幹細胞 / miRNA / 予後予測因子 / miR-137 / DKK1 / CD44 |
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| Outline of Final Research Achievements |
In this study, CD44 was found as a liver cancer stem cell marker associated with overall survival in patients with hepatocellular carcinoma (HCC). Furthermore, comparison of the gene and miRNA expression profiles between CD44-positive and CD44-negative HuH7 cells was performed. As a result of miRNA targeted pathway analysis of microarray data, pathway associated with the entire set of miRNA and mRNA including cell adhesion molecule, apoptosis, and ABC transporters in CD44-positive cells were different from those in CD44-negative cells, suggesting that CD44-positive liver cancer stem cell may associated with malignant progression in HCC patients. Additionally, DKK1 and miR-137, which were upregulated in CD44-positive liver cancer stem cell, were associated with poor prognosis of HCC patients and were an independent prognostic factor.
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Report
(4 results)
Research Products
(3 results)
