Elucidation of diagnostic procedure and the therapy that assumed microRNA and neovascularisation molecules a target in the hepatocarcinogenesis

• Project/Area Number: 26860510
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kagawa University
• Principal Investigator: Nomura Takako 香川大学, 医学部附属病院, 助教 (70645415)
• Research Collaborator: MASAKI Tsutomu
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肝細胞癌 / マイクロRNA / 血管新生分子

Outline of Final Research Achievements

We analyzed expression of miRNA in the hepatocellular carcinoma cell line cyclopedically, and clusters analyzed expression profiles after making and specifically identified miRNA s which caused expression changes in various kinds of hepatocellular carcinoma cell line.
Using the peripheral blood of patients with hepatocellular carcinoma given the radical operation of the hepatocellular carcinoma, we examined changes of miRNAs in the before and after therapy. The quantity of miRNA showed clear changes in before and after therapy and formed a different cluster. We significantly paid attention to miR-125a-5p as a candidate of a new tumor marker among 7 seen molecules of the decrease after treatment. It became clear that miR-125a-5p assumed molecules about cancer suppression such as the that strike of the cell cycle and the instruction of the apoptosis a target gene.

Research Products

• [Journal Article] MicroRNA profiles in cisplatin-induced apoptosis of hepatocellular carcinoma cells. (2015)
  • Author(s): Miyata M, Morishita A, Sakamoto T, Katsura A, Kato K, Nishioka T, Toyota Y, Fujita K, Maeda E, Nomura T, Tani J, Miyoshi H, Yoneyama H, Kobara H, Fujiwara S, Nishiyama N, Iwama H, Himoto T, Hirashima M, Masaki T.
  • Journal Title: Int J Oncol. Volume: 47 Issue: 2 Pages: 535-542
  • DOI: 10.3892/ijo.2015.3036
  • Peer Reviewed
• [Presentation] 肝細胞癌における新しい腫瘍マーカーとしての候補circulating microRNAの同定 (2016)
  • Author(s): 大浦杏子、米山弘人、正木勉
  • Organizer: 第52回日本肝臓学会総会
  • Place of Presentation: 千葉県
  • Year and Date: 2016-05-19