Epigenetic regulation of noncoding RNA in gastrointestinal stromal tumor
| Project/Area Number |
26860514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 消化管間質腫瘍 / エピジェネティクス / noncoding RNA / GIST |
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| Outline of Final Research Achievements |
We performed screening for detection of epigenetically silenced noncodingRNAs in GIST. For this purpose, GIST-T1 cells were treated by 5-aza-dc and 4-PBA. After which, expression of noncodingRNA and ChIP sequencing analysis were performed. We selected candidate genes which epigenetically silenced and found that miR-34a and miR-335 were frequently methylated in primary GIST. We also identified CpG island methylation of possible tumor suppressive lncRNA is associated with poor prognosis of GIST patients.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] A Screen for Epigenetically Silenced microRNA Genes in Gastrointestinal Stromal Tumors.2015
Author(s)
Isosaka M, Niinuma T, Nojima M, Kai M, Yamamoto E, Maruyama R, Nobuoka T, Nishida T, Kanda T, Taguchi T, Hasegawa T, Tokino T, Hirata K, Suzuki H, Shinomura Y
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Journal Title
PLoS One
Volume: 10
Issue: 7
Pages: e0133754-e0133754
DOI
Related Report
Peer Reviewed / Open Access
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