| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, we revealed molecular basis of laterally spreading tumor (LST), using genome-wide comprehensive analyses. Firstly, we assessed their epigenetic background. Subsequently, we conducted targeted exon sequencing including 38 candidate CRC driver genes. By hierarchical clustering using methylation information, LST was clearly classified into two subtypes; LST-G correlating to IME, and LST-NG correlating to LME. Genes associated with RTK/RAS signaling pathway were mutated more frequently in LST-G than LST-NG (P=0.004), especially KRAS mutation. Both LSTs showed high frequency of APC mutation even at adenoma stage, suggesting its involvement in initiation stage of LST, like adenoma-carcinoma sequence. TP53 mutation was specifically detected in cancer samples. TP53 mutation occurred during development of intramucosal cancer in LST-NG, but during development of cancer with submucosal invasion in LST-G, suggesting involvement of TP53 mutation at earlier stage in LST-NG.
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