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Mechanism of intestinal tumor formation through ADAM10

Research Project

Project/Area Number 26860528
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

Matano Mami  慶應義塾大学, 医学部, 研究員 (20439889)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords下部消化管学
Outline of Final Research Achievements

Our research group generated an epithelium specific ADAM10 knock-out mice through crossing ADAM10 flox mouse with villin-CreER mice. Tamoxifen induced knock out resulted in increased goblet cells and decreased intestinal stem cells. This can be explained by increased stem cell differentiation.
ADAM10 is involved in Notch signal activation through degradation of the intracellular domain. Indeed, ADAM10 knock-out intestinal epithelium inactivated Notch signal, resulting in loss of intestinal stem cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis2016

    • Author(s)
      Fujii M, Shimokawa M, Date S, Takano A, Matano M, Nanki K, Ohta Y, Toshimitsu K, Nakazato Y, Kawasaki K, Uraoka T, Toshiaki T, Watanabe Kanai T, Sato T.
    • Journal Title

      Cell Stem Cell

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.2016

    • Author(s)
      Mihara, E., Hirai, H., Yamamoto, H., Tamura-Kawakami, K., Matano, M., Kikuchi, A., Sato, T., and Takagi, J.
    • Journal Title

      Elife

      Volume: 5 Pages: 1-19

    • DOI

      10.7554/elife.11621

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient genetic engineering of human intestinal organoids using electroporation.2015

    • Author(s)
      Fujii M, Matano M, Nanki K, Sato T.
    • Journal Title

      Nature Protocols

      Volume: 10 Issue: 10 Pages: 1474-85

    • DOI

      10.1038/nprot.2015.088

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sweetness characterization of recombinant human lysozyme.2015

    • Author(s)
      Matano M, Nakajima K, Kashiwagi Y, Udaka S, Maehashi K.
    • Journal Title

      Comp Biochem Physiol B Biochem Mol Biol.

      Volume: 188 Pages: 8-14

    • DOI

      10.1016/j.cbpb.2015.05.009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modelling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids.2015

    • Author(s)
      Matano M,Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T.
    • Journal Title

      Nature Medicine

      Volume: 21(3) Issue: 3 Pages: 256-262

    • DOI

      10.1038/nm.3802

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cross-talk between RORgammat+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.2014

    • Author(s)
      1.Mizuno S, Mikami Y, Kamada N, Handa T, Hayashi A, Sato T, Matsuoka K, Matano M, Ohta Y, Sugita A, Koganei K, Sahara R, Takazoe M, Hisamatsu T, Kanai T.
    • Journal Title

      Inflamm Bowel Dis

      Volume: 20(8) Issue: 8 Pages: 1426-1434

    • DOI

      10.1097/mib.0000000000000105

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biochemical and functional characterization of transiently expressed in neural precursor (TENP) protein in emu egg white.2014

    • Author(s)
      Maehashi K, Ueda M, Matano M, Takeuchi J, Uchino M, Kashiwagi Y, Watanabe T.
    • Journal Title

      J Agric Food Chem.

      Volume: 62(22) Issue: 22 Pages: 5156-62

    • DOI

      10.1021/jf5008117

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] The Self-renewing Mechanism of Intestinal Stem Cell: Niche and Cancer2014

    • Author(s)
      Toshiro Sato, Yuki Ohta, Ai Takano, Shoichi Date, Mamiko Matano, Mariko Shimokawa.
    • Organizer
      7th Annual Meeting of the Japanese Society of Developmental Biologists and Asia Pacific Developmental Biology Network
    • Place of Presentation
      WINC愛知(名古屋)
    • Year and Date
      2014-05-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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