Impact of clinical characteristics on the yield of therapeutic adipose-derived mesenchymal stem cells in a cardiovascular disease

• Project/Area Number: 26860549
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kanazawa University
• Principal Investigator: Takashima Shinichiro 金沢大学, 医薬保健学総合研究科, 特任助教 (60547165)
• Co-Investigator(Kenkyū-buntansha): INOUE Oto 金沢大学, 附属病院循環器内科, 医員 (00781147)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 間葉系幹細胞 / 皮下脂肪 / 心血管疾患 / 脂肪由来間葉系幹細胞 / 心臓再生治療

Outline of Final Research Achievements

The problem of autologous stem cell therapy for cardiovascular disease is that a large variability of therapeutic effects among patients. We conducted a clinical study to examine the variation in yield of therapeutic stem cells in subcutaneous adipose tissue (SAT) among patients. From 17 patients who underwent cardiac surgery, 1-2g of SAT was obtained and digested with collagenase to isolate stromal vascular fraction (SVF). Mesenchymal stem cell markers in SVF were analyzed by flow cytometry. The frequency of the patients with hypertension, dyslipidemia and diabetes was 94%, 77% and 59%, respectively. Interestingly, the content of CD271-positive cells that is reported as a specific marker of therapeutic stem cells largely varied among patients and was associated with the specific clinical feature. These indicated that the clinical background would impact on yield of therapeutic stem cells in SAT and efficacy of cardiac stem cell therapy.

Research Products

• [Presentation] 脂肪組織慢性炎症が間葉系幹細胞プロファイリングに及ぼす影響 (2016)
  • Author(s): 井上己音、高島伸一郎、薄井荘一郎、杉田光洋、加藤武史、村井久純、古荘浩司、酒井佳夫、竹村博文、金子周一、高村雅之
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-19
• [Presentation] Insulin resistance predicts the yield of therapeutic stem cells in adipose tissue for autologous grafts in patients with cardiovascular disease (2016)
  • Author(s): Oto Inoue, Shinichiro Takashima, Soichiro Usui, Mitsuhiro Sugita, Takeshi Kato, Hisayoshi Murai, Hiroshi Furusho, Hirofumi Takemura, Yoshio Sakai, Shuichi Kaneko, Masayuki Takamura
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-03-18
• [Presentation] Adipose tissue-derived stream cells exert beneficial effect to systemic response following myocardial ischemia reperfusion (2016)
  • Author(s): Usui soichiro, Shinichiro Takashima, Oto Inoue, Hirochi Ootsuji, Hirayoshi Murai, Shuichi Kaneko, Masayuki Takamura
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-03-18
• [Presentation] 脂肪由来間葉系細胞投与による心筋虚血再灌流後の全身応答への影響 (2016)
  • Author(s): 薄井荘一郎、高島伸一郎、井上己音、酒井佳夫、高村雅之、金子周一
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17