Development of cardiac regenerative drug therapy recruiting endogenous cardiac stem/progenitor cells

• Project/Area Number: 26860555
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kyoto University
• Principal Investigator: Fukushima Hiroyuki 京都大学, 学内共同利用施設等, 研究員 (10713860)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 心臓再生 / ケミカルバイオロジー / ES/iPS細胞 / 心臓再生治療 / 心筋細胞 / 低分子化合物 / 再生医療

Outline of Final Research Achievements

Previously, we identified CDC1 which induced cardiomyocyte differentiation from ES/iPS cells. This study aimed development of cardiac regenerative drug therapy with utilizing CDC1. Following term were investigated; 1) Structure-activity relationship among CDC1 analogs, 2) Identification of compounds having synergistic effect with CDC1, 3) Development of gelatin hydrogel particle containing CDC1. We successfully identified some analogs showing same effect, comparing with CDC1. Although, we could not identify a new synergistic effect compound, fortunately we obtained a compound, which was hopeful drug for therapeutic use, unlike CDC1. Furthermore we are investigating utility of gelatin hydrogel particle containing CDC1.

Research Products

• [Journal Article] Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration. (2014)
  • Author(s): Masumoto H, Ikuno T, Takeda M, Fukushima H, Marui A, Katayama S, Shimizu T, Ikeda T, Okano T, Sakata R, Yamashita JK.
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 6716-6716
  • DOI: 10.1038/srep06716
  • Peer Reviewed / Open Access
• [Presentation] Identification of novel chemicals potently inducing cardiomyocyte differentiation from mouse and human pluripotent stem cells (2014)
  • Author(s): Fukushima H
  • Organizer: International Society for Stem Cell Research 12th Annual Meeting
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2014-06-18 – 2014-06-21
• [Remarks] 山下研究室
  • URL: http://www.frontier.kyoto-u.ac.jp/es02/index.htm