| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the potential to serve as a tool to investigate human development/differentiation. We modified our vascular differentiation system of human ES/iPS cells and we have induced vascular cells more effectively and stably from various ES/iPS cell clones without any feeder cells.
Using the iPS cells from patients with genetic abnormalities (patient-specific iPS cells) in vessels, we can observe vascular differentiation/development in vitro and identify new susceptibility genes associated with vascular abnormalities. Applying our differentiation system for Moyamoya-iPS, ADPKD-iPS and Takayasu-iPS cells, we have identified several molecules whose expression levels were upregulated or downregulated in vascular cells differentiated from patient-specific-iPSCs as compared to those from normal Japanese iPSCs. These results would contribute to a novel understanding of vascular abnormalities in each disease.
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