The establishment of vascular cell differentiation system from human ES/iPS cells and its application for the clarification of vascular pathology
Project/Area Number |
26860557
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Cardiovascular medicine
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Research Institution | Kyoto University |
Principal Investigator |
Taura Daisuke 京都大学, 医学(系)研究科(研究院), 助教 (10558612)
|
Research Collaborator |
Masakatsu Sone
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 血管細胞誘導 / iPS細胞 |
Outline of Final Research Achievements |
Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the potential to serve as a tool to investigate human development/differentiation. We modified our vascular differentiation system of human ES/iPS cells and we have induced vascular cells more effectively and stably from various ES/iPS cell clones without any feeder cells. Using the iPS cells from patients with genetic abnormalities (patient-specific iPS cells) in vessels, we can observe vascular differentiation/development in vitro and identify new susceptibility genes associated with vascular abnormalities. Applying our differentiation system for Moyamoya-iPS, ADPKD-iPS and Takayasu-iPS cells, we have identified several molecules whose expression levels were upregulated or downregulated in vascular cells differentiated from patient-specific-iPSCs as compared to those from normal Japanese iPSCs. These results would contribute to a novel understanding of vascular abnormalities in each disease.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Impaired adipogenic capacity in induced pluripotent stem cells from lipodystrophic patients with BSCL2 mutations2016
Author(s)
1.E. Mori, J. Fujikura, M. Noguchi, K. Nakao, M. Matsubara, M. Sone, D. Taura, T. Kusakabe, K. Ebihara, T. Tanaka, K. Hosoda, K. Takahashi, I. Asaka, N. Inagaki, K. Nakao
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Journal Title
Metablism
Volume: 65
Issue: 4
Pages: 543-556
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Biochemical and functional characterization of RNF213(Mysterin) R4810K, a susceptibility mutation of moyamoya disease, in angiogenesis in vitro and in vivo.2015
Author(s)
Kobayashi H, Matsuda Y, Hitomi T, Okuda H, Shioi H, Matsuda T, Imai H, Sone M, Taura D, Harada KH, Habu T, Takagi Y, Mimyamoto S, Koizumi A.
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Journal Title
Journal of the American Heart Association
Volume: Jun 30;4(7)
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Role of endogenous ACTH on circadian aldpsterone rhythm in patients with primary aldosteronism.2014
Author(s)
Sonoyama T, Sone M, Tamura N, Honda K, Taura D,Kojima K,Fukuda Y,Kanamono N, Miura M, Yasoda A, Arai H, Itoh H, Nakao K.
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Journal Title
Endocr.Connect.
Volume: Dec3(4)
Pages: 173-179
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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