Interaction of Renin-Angiotensin System with microRNA in Vascular Senescence

• Project/Area Number: 26860567
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Ehime University
• Principal Investigator: Min Li-Juan 愛媛大学, 医学部附属病院, 助教(病院教員) (80726175)
• Co-Investigator(Kenkyū-buntansha): HORIUCHI Masatsugu 愛媛大学, 大学院医学系研究科, 教授 (40150338) ; MOGI Masaki 愛媛大学, 大学院医学系研究科, 准教授 (20363236)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 動脈硬化 / 血管老化 / レニン・アンジオテンシン・アルドステロン系 / microRNA-21 / Ras/MAPK シグナル / 血管平滑筋細胞

Outline of Final Research Achievements

We examined the possible role of microRNA-21, which involved in Ras/ERK signaling pathway and its target gene Spry1, in angiotensin II-induced vascular senescence. In vitro, we observed that microRNA-21 was associated with angiotensin II-induced VSMC senescence together with its downstream target molecule. In vivo, we did not obtained the same results in this time. In future, it is necessary to investigate the interaction of microRNA-21 with vascular senescence using other senescent mouse models. We have not yet observed the possible involvement of Spry1 in the microRNA-21 associated VSMC senescence. In future, the examination of other molecules such as Sp-1 is needed.

Research Products

• [Journal Article] Angiotensin II Type 2 Receptor Inhibits Vascular Intimal Proliferation With Activation of PPARγ (2015)
  • Author(s): Kukida M, Mogi M, Ohshima K, Nakaoka H, Iwanami J, Kanno H, Tsukuda K, Chisaka T, Min LJ, Wang XL, Bai HY, Shan BS, Higaki A, Yamauchi T, Okura T, Higaki J, Horiuchi M
  • Journal Title: Am J Hypertens Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 柑橘ジュース飲用により血管リモデリングが抑制される (2015)
  • Author(s): 閔莉娟、浅山理恵、大西亜里香、茂木正樹、中岡裕智、菅野晴美、佃架奈、莖田昌敬、山内俊史、檜垣彰典、王小俐、白薈鈺、単宝帥、岩波純、堀内正嗣
  • Organizer: 第38回日本高血圧学会総会
  • Place of Presentation: （ひめぎんホール) 愛媛県松山市 日本
  • Year and Date: 2015-10-09
• [Presentation] アンジオテンシンII2型受容体刺激は一部ATIPを介したPPAR-gammaの活性化により血管リモデリングを抑制する (2015)
  • Author(s): 莖田昌敬、茂木正樹、中岡裕智、檜垣彰典、単宝帥、白薈鈺、菅野晴美、佃架奈、王小俐、閔莉娟、岩波純、大蔵隆文、檜垣實男、堀内正嗣
  • Organizer: 第38回日本高血圧学会総会
  • Place of Presentation: （ひめぎんホール) 愛媛県松山市 日本
  • Year and Date: 2015-10-09
• [Presentation] 子宮内胎児発育遅延は血管リモデリングを促進させるリスクとなる (2015)
  • Author(s): 山内俊史、茂木正樹、千阪俊行、中岡裕智、莖田昌敬、菅野晴美、佃架奈、王小俐、白薈鈺、単宝帥、閔莉娟、岩波純、檜垣高史、堀内正嗣
  • Organizer: 第38回日本高血圧学会総会
  • Place of Presentation: （ひめぎんホール) 愛媛県松山市 日本
  • Year and Date: 2015-10-09
• [Remarks] 愛媛大学大学院医学系研究科 分子心血管生物・薬理学
  • URL: http://www.m.ehime-u.ac.jp/school/biochem1