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Identification of DHRS7C for aggravation factor in cardiomyopathy

Research Project

Project/Area Number 26860570
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cardiovascular medicine
Research InstitutionKyushu University

Principal Investigator

Arai Shinobu  九州大学, 学内共同利用施設等, 研究員 (30529970)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsDHRS7C / ER/SR / calcium / hypertrophy / カルシウム / 筋小胞体 / カルパイン / 小胞体 / カルシウム調節
Outline of Final Research Achievements

DHRS7C is a molecule specifically expressed in heart and skeletal muscle, of which functions remain unclear. DHRS7C decreases its mRNA expression in myocardium in accordance with LV systolic dysfunction. Therefore we aimed to characterize the biological role of DHRS7C using C2C12. We generated cell lines with DHRS7C knock and point mutations in NAD/NADH dehydrogenase core domain of DHRS7C, YXXXK to Y191F and K195Q, in C2C12. We examined cellular morphology, calcium influx/release using fura2, and calpain activity.We found DHRS7C transcription was increased when they were differentiated in C2C12 and showed localization in SR. DHRS7C-KO and two mutant lines showed cellular hypertrophy, and extracellular calcium influx and ER calcium release were also increased significantly compared to control.DHRS7C regulates intracellular Ca2+ homeostasis depending on NAD/NADH dehydrogenase activity, and functional loss of DHRS7C causes abnormal Ca2+ increase and cellular hypertrophy.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Overexpression of TFAM or Twinkle Increases mtDNA Copy Number and Facilitates Cardioprotection Associated with Limited Mitochondrial Oxidative Stress.2015

    • Author(s)
      Ikeda M, Ide T, Fujino T, Arai S, Saku K, Kakino T, Tyynismaa H, Yamasaki T,Yamada K, Kang D, Suomalainen A, Sunagawa K.
    • Journal Title

      PLoS One.

      Volume: 10 Issue: 3 Pages: e0119687-e0119687

    • DOI

      10.1371/journal.pone.0119687

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Muscle specific NAD/NADH dehydrogenase, DHRS7C, maintains intracellular Ca2+ homeostasis and cellular morphology.2015

    • Author(s)
      新井 しのぶ
    • Organizer
      The 32nd Annual Meeting of the ISHR Japanese Section
    • Place of Presentation
      横浜
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis2015

    • Author(s)
      新井 しのぶ
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis and cell morphology.2014

    • Author(s)
      Arai S, Ide T, Ikeda M, Hirano K, Matsuo Y, Sunagawa K
    • Organizer
      The 31st Annual Meeting of the international society for Heart Research Japanese Section
    • Place of Presentation
      愛知県
    • Year and Date
      2014-11-28 – 2014-11-29
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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