MURC/Cavin-4 deficiency exacerbates abdominal aortic aneurysm
| Project/Area Number |
26860580
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Miyagawa Kotaro 京都府立医科大学, 医学(系)研究科(研究院), 助教 (60725612)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腹部大動脈瘤 |
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| Outline of Final Research Achievements |
We showed the role of MURC (muscle-restricted coiled-coil protein)/Cavin-4 in vascular smooth muscle cells (VSMCs) and AAA development. Wild-type and MURC knockout (MURC-/-) mice were subjected to periaortic application of CaCl2 to induce AAA. Six weeks after CaCl2 treatment, internal and external aortic diameters were significantly increased in MURC-/- AAA compared with WT AAA, which was accompanied by increased fibrosis in the tunica media of MURC-/- AAA. JNK and matrix metalloproteinase-2 activities were increased in MURC－/－ AAA compared with WT AAA. TNFα-induced JNK activation was enhanced in MURC-knockdown VSMCs compared with control VSMCs. Furthermore, WT, MURC-/-, apolipoprotein E-/- (ApoE-/-), and MURC and ApoE double-knockout (MURC-/-ApoE-/-) mice were subjected to angiotensin II infusion. Four weeks after angiotensin II infusion, the internal aortic diameter was significantly increased in MURC-/-ApoE-/- mice compared with ApoE-/- mice and MURC-/- mice.
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Report
(3 results)
Research Products
(1 results)
